Potential lipase inhibitor for obesity treatment

Alias, Norsyuhada and Leow, Thean Chor and Mohammad Ali, Mohd Shukuri and Salleh, Abu Bakar and Ahmad Tajudin, Asilah and Raja Abd.Rahman, Raja Noor Zaliha (2015) Potential lipase inhibitor for obesity treatment. In: Science and Business 2nd Joint Symposium of Universiti Putra Malaysia and University of Hyogo, 22 - 23 Oct 2015, University of Hyogo, Japan.

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Abstract

Pancreatic lipase inhibitors from natural resources are a potential tool for the treatment of obesity. The inhibitory activity of 24 crude extracts of selected Malaysian plants against porcine pancreatic lipase (PPL) was successfully screened, in vitro. Orthosiphon stamineus, Phyllanthus niruri, Murraya paniculata and Averrhoa bilimbi leaves extracts showed more than 70% inhibitions when incubated with PPL at a final concentration of 500 µg/mL for 15 minutes at 37⁰C. P. niruri crude extract exhibited strongest lipase inhibitory activity, with an IC50 value of 27. 65 µg/mL followed by O. stamineus, M. paniculata and A. bilimbi with IC50 values of 34.74, 41.45 and 55.18 µg/mL, respectively. The inhibition mode study disclosed that P. niruri could act as noncompetitive inhibitor. Antioxidant activity of P. niruri extract was measured by 2, 2, diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity assay. P. niruri showed high antioxidant activity with an EC50 value of 7.471 µg/mL. The results suggested that P. niruri has shown potential as a source of natural antilipase and antioxidant.

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	7690/53182
Uncontrolled Keywords:	Pancreatic lipase inhibitors, obesity, Phyllanthus niruri, antilipase, antioxidant, DPPH
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User:	Norsyuhada Alias
Date Deposited:	28 Dec 2016 19:39
Last Modified:	28 Dec 2016 19:39
URI:	http://irep.iium.edu.my/id/eprint/53182

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