Extraction optimization and properties of collagen from chicken (Gallus gallus domesticus) feet

Mokhtar, Nur Dhiya and Abdul Wahab, Widya and Ab. Hadi, Hazrina and Abu Hassan, Muhamad Shafiq and Haron, Normah and Muhamad Bunnori, Noraslinda (2016) Extraction optimization and properties of collagen from chicken (Gallus gallus domesticus) feet. In: Medical Research Symposium (MRS) and Pacific Partnership in Conjunction with Kuantan Research Day 2016, 4 August 2016, Kulliyyah of Medicine, IIUM Kuantan. (Unpublished)

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Abstract

Introduction: Collagen can be basically extracted by chemical and enzymatic hydrolysis. In many industries, chemical hydrolysis method is more commonly used. However, the enzymatic hydrolysis is more effective when the high nutritional value product will be produced based on the extracted material. Chicken (Gallus gallus domesticus) feet were used as a source of bird collagen. Method: Optimization of collagen extraction was investigated by using three different methods which were soaked in 5% lactic acid for 36 hours, 0.1% papain in 0.5 mol/L acetic acid and 0.1% bromelain in 0.5 mol/L acetic acid. Various properties such as percentage of end yield, concentration of protein, pH, swelling percentage and SDS-PAGE patterns of collagen from chicken feet were evaluated in order to determine the best method of extraction. Result: Type I collagen was considered as major component of chicken feet collagen which indicated by β chain and two distinct α-chains which are α1 and α2 that observed from electrophoresis pattern of collagen extracted from all three methods. Lactic acid method showed the highest percentage of end yield and concentration of protein compared to another two methods. However, lactic acid method showed the lowest swelling percentage and highest pH values. Conclusion: According to these results, soaked in 5% lactic acid for 36 hours is possible as the best collagen extraction method from chicken feet.

Item Type:	Conference or Workshop Item (Speech/Talk)
Additional Information:	6604/52589
Uncontrolled Keywords:	Chicken feet, collagen, lactic acid, papain, bromelain, SDS Page
Subjects:	S Agriculture > S Agriculture (General) S Agriculture > SB Plant culture
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Dr. Noraslinda Muhamad Bunnori
Date Deposited:	28 Apr 2017 11:51
Last Modified:	13 Jul 2017 15:26
URI:	http://irep.iium.edu.my/id/eprint/52589

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