Proteomic characterization and comparison of Malaysian Tropidolaemus wagleri and Cryptelytrops purpureomaculatus venom using shotgun-proteomics

Zainal Abidin, Syafiq Asnawi and Rajadurai, Pathmanathan and Chowdhury, Md Ezharul Hoque and Ahmad Rusmili, Muhamad Rusdi and Othman, Iekhsan and Naidu, Rakesh (2016) Proteomic characterization and comparison of Malaysian Tropidolaemus wagleri and Cryptelytrops purpureomaculatus venom using shotgun-proteomics. Toxins, 8 (10). 299-1-299-14. ISSN 2072-6651

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Abstract

Tropidolaemus wagleri and Cryptelytrops purpureomaculatus are venomous pit viper species commonly found in Malaysia. Tandem mass spectrometry analysis of the crude venoms has detected different proteins in T. wagleri and C.purpureomaculatus. They were classified into 13 venom protein families consisting of enzymatic and nonenzymatic proteins. Enzymatic families detected in T. wagleri and C. purpureomaculatus venom were snake venom metalloproteinase, phospholipase A2, L-amino acid oxidase, serine proteases, 50-nucleotidase, phosphodiesterase, and phospholipase B. In addition, glutaminyl cyclotransferase was detected in C. purpureomaculatus. C-type lectin-like proteins were common nonenzymatic components in both species. Waglerin was present and unique to T. wagleri—it was not in C. purpureomaculatus venom. In contrast, cysteine-rich secretory protein,bradykinin-potentiating peptide, and C-type natriuretic peptide were present in C. purpureomaculatus venom. Composition of the venom proteome of T. wagleri and C. purpureomaculatus provides useful information to guide production of effective antivenom and identification of proteins with potential therapeutic applications.

Item Type:	Article (Journal)
Additional Information:	5591/52355
Uncontrolled Keywords:	Tropidolaemus wagleri; Cryptelytrops purpureomaculatus; proteome; tandem mass spectrometry
Subjects:	Q Science > QL Zoology R Medicine > RM Therapeutics. Pharmacology R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Basic Medical Sciences
Depositing User:	Dr Muhamad Rusdi Ahmad Rusmili
Date Deposited:	19 Oct 2016 09:00
Last Modified:	21 Oct 2017 19:02
URI:	http://irep.iium.edu.my/id/eprint/52355

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