Multi-step enzyme reaction in an Immobilized Enzyme Microreactor (IEMR)

Abdul Halim, Amanatuzzakiah and Baganz, Frank (2016) Multi-step enzyme reaction in an Immobilized Enzyme Microreactor (IEMR). In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur.

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Abstract

The application of biocatalyst in microfluidic systems has attracted huge attention in recent years due to their potential for creating microscale analytical tools with small volumes, high-throughput and attractive prospect for mass production. This system can be integrated with the multi-step enzyme reaction to synthesize complex organic compounds such as a single diastereoisomer of 2-amino-1,3,4-butanetriol (ABT). ABT is an interesting compound, commonly used as the building block in several pharmaceutical syntheses. In this work we study a de novo designed pathway for the production of ABT using IEMRs, which is based on the reversible attachment of His6-tagged enzymes via Ni-NTA agarose beads that are packed into tubes to enable continuous flow of multi-step enzyme reactions. The synthesis of ABT was demonstrated in a three-step enzyme reaction using two enzymes; transketolase and transaminase. In the first part of the three-step reaction, His6-TAm catalysed the conversion of the substrates, serine (SER) and glycoaldehyde (GA), to form lithium-hydroxypyruvate (HPA). The product of the first reaction was then continuously fed to the second IEMR containing the immobilized His6-TK, which catalysed the reaction between HPA and GA into erythrulose (ERY). The multi-step reaction was completed with the amination of ERY by the second His6- TAm to produce the final target product, ABT.

Item Type:	Conference or Workshop Item (Plenary Papers)
Additional Information:	5355/52183
Uncontrolled Keywords:	biocatalyst; microfluidic; Immobilized Enzyme Microreactor (IEMR
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	ms Amanatuzzakiah Abdul Halim
Date Deposited:	13 Oct 2016 16:35
Last Modified:	13 Oct 2016 16:35
URI:	http://irep.iium.edu.my/id/eprint/52183

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