Potential inhibitors of α-glucosidase and α-amylase enzymes from local fruit waste by solid state fermentation

Ahmad Barkat, Anumsima and Jamal, Parveen and Noor Batcha, Ibrahim Ali and Zainudin, Zaki and Suhaida Azmi, Azlin and Arbain, Dachyar and Mohamed Idris, Zulkarnain (2016) Potential inhibitors of α-glucosidase and α-amylase enzymes from local fruit waste by solid state fermentation. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur, Malaysia.

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Abstract

One therapeutic approach for treating diabetes is to decrease the post-prandial hyperglycaemia. This is done by retarding the absorption of glucose through the inhibition of the carbohydrate hydrolyzing enzymes, α- amylase and α-glucosidase, in the digestive tract. Inhibition of both enzymes will help to reduce glucose level in the blood of diabetic patient. This study was aimed to investigate the production of α-glucosidase and α- amylase inhibitors from local fruit wastes (honeydew skin, banana peel, and pineapple skin) using solid state fermentation. Each of the fruit waste was fermented with three different types of white rot fungus (Phenarochaete chrysosporium (P.chrysosporium), Panus tigrinus MQR609 (M6) and RO2) for 7 days. Sampling was done starting from day 4 to day 7 to determine enzyme inhibition activity. The samples were extracted using water prior to enzyme analysis. Most of the fruit samples showed varying degrees of percentage inhibition activity depending on the sampling time. Extract of fermented banana peels with RO2 on day 4 showed higher potential of α-glucosidase inhibition (56.57±0.32%). Followed with honeydew extract fermented with the same fungus on the same day showed 39.68±0.05%. Extracts of each fruit wastes sample fermented with P.chrysosporium showed least potential of α-glucosidase inhibition (below 15%). While for α- amylase inhibition activity the extract from fermented honeydew skins with P.chrysosporium on day 7 showed the highest potential inhibition activity 98.29±0.63%. The least potential inhibition activity occurred in extract from honeydew skins fermented with M6 on day 5 showed 43.37±0.54%. All the positive result showed that fruit waste can be alternatives sources for antidiabetic agent especially for α-amylase and α- glucosidase inhibitors.

Item Type:	Conference or Workshop Item (Invited Papers)
Additional Information:	2937/51650
Uncontrolled Keywords:	diabetes, hyperglycaemia, α-glucosidase, α-amylase , Phenarochaete chrysosporium
Subjects:	R Medicine > RC Internal medicine T Technology > T Technology (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Prof.Dr. Parveen Jamal
Date Deposited:	16 Aug 2016 16:05
Last Modified:	16 Aug 2016 16:05
URI:	http://irep.iium.edu.my/id/eprint/51650

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