Effect of abiotic stress on carotenoids accumulation in pumpkin plants and callusses under light and dark conditions

Shahidan, Norshazila and Othman, Rashidi and Jaswir, Irwandi and Hashim, Yumi Zuhanis Has-Yun (2016) Effect of abiotic stress on carotenoids accumulation in pumpkin plants and callusses under light and dark conditions. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur.

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Abstract

Pumpkin is abundantly planted in Malaysia and very rich with carotenoid compounds that can support healthy living due to its antioxidant properties. However, the research on its carotenoid compound has not received the attention it deserves, either from researchers or government. This research was conducted to study the effect of abiotic stress on the carotenoids accumulation through in pumpkin plants and calluses under light and dark conditions. The abiotic stresses used in this study were Ultra Violet light exposure, Polyethylene Glycol 4000, Jasmonic Acid, Salicylic Acid and Murashige and Skoog Salt. It was found that exposure to Ultra Violet light could improve the purity of lutein compound in pumpkin leaves with the absence of β-carotene. Salicylic Acid treatment under light condition was found to improve the lutein compound to ten times higher than the controlled treatment in pumpkin stems. The highest yield of β-carotene compound in pumpkin calluses was detected in half strength treatment under light condition. Even though the carotenoid compound in pumpkin plants and calluses could be improved with the treatments mentioned above, the mechanisms involved regarding the treatment effects on carotenoid biosynthesis has not been well studied. Thus, further study should be conducted to understand the mechanisms better.

Item Type:	Conference or Workshop Item (Plenary Papers)
Additional Information:	3842/51647 (ISBN: 978-983-42978-8-6)
Uncontrolled Keywords:	Carotenoid, Biogenesis manipulation, pumpkin plants, pumpkin calluses
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	DR YUMI ZUHANIS HAS-YUN HASHIM
Date Deposited:	17 Aug 2016 11:10
Last Modified:	13 Jan 2017 16:11
URI:	http://irep.iium.edu.my/id/eprint/51647

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