Cytotoxic, anti-inflammatory and adipogenic effects of inophyllum D, calanone,isocordato-oblongic acid, and morelloflavone on cell lines

Bakhtiar, M. Taher and Aminuddin, Amnani and Darnis, Deny Susanti and Aminudin, Nurul Iman and On, Shamsul and Ahmad, Farediah and Hamidon, Hanisuhana (2016) Cytotoxic, anti-inflammatory and adipogenic effects of inophyllum D, calanone,isocordato-oblongic acid, and morelloflavone on cell lines. Natural Products Sciences, 22 (2). pp. 122-128. ISSN 1226-3907 E-ISSN 2288-9027

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Abstract

This paper reports in vitro cytotoxic, anti-inflammatory and adipocyte diffentiation with adipogenic effects of coumarins inophyllum D (1) and calanone (2), and a chromanone carboxylic acid namely isocordatooblongic acid (3) isolated from Calophyllum symingtonianum as well as a biflavonoid morelloflavone (4) isolated from Garcinia prainiana on MCF-7 breast adenocarcinoma RAW 264.7 macrophages and 3T3-L1 preadipocytes cells, respectively. The cytotoxicity study on MCF-7 cell was conducted by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay. Meanwhile, the study of anti-inflammatory effects in RAW 264.7 macrophages and adipogenic effects on 3T3-L1 pre-adipocytes were conducted through nitrite determination assay and induction of adipocyte differentiation, respectively. In the cytotoxicity study, inophyllum D (1) was the only compounds that exhibited significant cytotoxic effect against MCF-7 cell with IC50 of 84 μg/mL. Further, all by inhibiting the compounds have shown anti-inflammatory effects in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages of nitrite concentration with production. In addition, the compounds also exhibited adipogenic effects on 3T3-L1 pre-adipocytes by stimulating lipid formation. Thus, this study may provide significant input in discovery of the potential effects cytotoxic, anti-inflammatory and adipogenic agents.

Item Type:	Article (Journal)
Additional Information:	5209/51411
Uncontrolled Keywords:	Cytotoxic, Anti-inflammatory, Adipogenic
Subjects:	R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Technology Kulliyyah of Science > Department of Biomedical Science
Depositing User:	Dr Muhammad Taher
Date Deposited:	04 Aug 2016 15:52
Last Modified:	10 Apr 2017 15:57
URI:	http://irep.iium.edu.my/id/eprint/51411

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