Bioethanol production from sago starch

Mel, Maizirwan and Muhammad Nadzri, Husna and Kamarudin, Mohd Hider and Abd Karim, Mohd Ismail (2010) Bioethanol production from sago starch. In: International Conference on Technology for New and Renewable Energy (ICT-NRE), 1 - 3 December, 2010, Jakarta Indonesia.

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Abstract

Two stage processes prior to ethanol production has been proposed which is hydrolysis and fermentation. Commercial enzymes were used in this two steps hydrolysis; α-amylase (liquefaction step) and glucoamylase (saccharification step). Optimization was carried out in both stages; hydrolysis and fermentation. Three parameters are involved in optimization of % dextrose equivalent (DE); sago starch concentration (20% (w/v), 30% (w/v), 40% (w/v)), glucoamylase enzyme (52 U/g, 78 U/g, 104 U/g) and time during saccharification (1, 2, 3 hours). Three parameters are involved in optimization of ethanol; agitation (100 rpm, 150 rpm, 200 rpm), inoculums (1% (v/v), 3% (v/v), 5% (v/v)) from constant initial stock of 2.5x106 cells/mL and pH (4, 5, 6). Both optimization studies were carried out using Box-Behnken design. The experiment showed that the optimum parameters for hydrolysis study was identified to be glucoamylase (75.87 U/g), substrate concentration (28.49% (w/v)), and time (2 hours) which produced 62.15 g/L glucose as the fermentation substrate. For ethanol fermentation study, it was identified that the optimum parameters that produced 29.25 g/L were 167 rpm agitation, 3.43% (v/v) inoculums and pH 5.

Item Type:	Conference or Workshop Item (Full Paper)
Additional Information:	4085/5044 (978-602-99040-0-0)
Uncontrolled Keywords:	Sago; fermentation; hydrolysis; ethanol; optimization
Subjects:	T Technology > TP Chemical technology > TP155 Chemical engineering T Technology > TP Chemical technology > TP248.13 Biotechnology T Technology > TP Chemical technology > TP315 Fuel T Technology > TP Chemical technology > TP500 Fermentation industries. Beverages. Alcohol
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr Maizirwan Mel
Date Deposited:	25 Nov 2011 14:18
Last Modified:	27 Feb 2012 17:01
URI:	http://irep.iium.edu.my/id/eprint/5044

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