In Vitro and In Vivo wound healing studies of standardized extract of Centella asiatica

Abdul Aziz, Huwaida and Bakhtiar, Muhammad Taher and Sh Ahmed, Afnan and Wan Sulaiman, Wan Mohd. Azizi and Darnis, Deny Susanti and Roy Chowdhury, Shiplu and Ahmad, Farediah and Zakaria, Zainul Amiruddin (2016) In Vitro and In Vivo wound healing studies of standardized extract of Centella asiatica. In: International Conference on Natural Products 2016 (ICNP 2016), 15th-17th March 2016, Kuala Terengganu, Terengganu, Malaysia. (Unpublished)

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Abstract

The current study evaluates the wound healing efficacy of Centella asiatica via in vitro scratch assay and in vivo circular wound excision model. The ethanol extract was qualitatively identified using thin layer chromatography and the positive fraction containing asiaticoside was further quantified using reverse-phase HPLC. The asiaticoside rich fraction was subjected to: i) colorimetric MTT (methylthiazoltetrazolium) cell viability study following incubation with human dermal fibroblast (HDF) and human dermal keratinocyte (HaCaT); ii) in vitro 12-well plate scratch assay (using HDF and HaCaT cells); iii) topical application (40, 10, and 2.5%, w/w) on in vivo circular wound excision of rabbits. Data on wound contraction, epithelization period, hydroxyproline content and histophatological analysis was collected from in vivo study. The result showed that the methanol fraction of extract contained about 2.4% of asiaticoside. Based on the results of colorimetric MTT (methylthiazoltetrazolium) cell viability assay, both HDF and HaCaT showed significant stimulation upon application of the methanolic fraction of extract at concentrations of 100 µg/mL and 0.19 µg/mL. In the scratch assay, methanol fraction of extract with concentration of 0.2 µg/mL and 100 µg/mL showed significant effect on HDF and HaCaT compared to the positive control (P< 0.05). In vivo, it was shown that the methanol fraction of the standardized extract induced collagen synthesis. Histopathology data also concluded that dose-dependent effect of the tested extract as a wound healing was present. In conclusion, the claim on the presence of wound healing properties in C. asiatica had been well supported based on the results obtained in this study.

Item Type:	Conference or Workshop Item (Slide Presentation)
Additional Information:	5209/50094
Uncontrolled Keywords:	Centella asiatica, asiaticoside, scratch assay, circular excision wound
Subjects:	R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Basic Medical Sciences Kulliyyah of Pharmacy > Department of Pharmaceutical Technology Kulliyyah of Science > Department of Chemistry
Depositing User:	Dr Muhammad Taher
Date Deposited:	12 Apr 2016 14:28
Last Modified:	05 Dec 2016 01:04
URI:	http://irep.iium.edu.my/id/eprint/50094

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