Preparation, characterization and in vitro release study of BSA-loaded double-walled glucose-poly(lactideco- glycolide) microspheres

Ansary, Rezaul Haque and Rahman, Md. Mokhlesur and Awang, Mohamed and Katas, Haliza and Ab. Hadi, Hazrina and Mohamed, Farahidah and Doolaanea, Abd Almonem and Yunus, Kamaruzzaman (2016) Preparation, characterization and in vitro release study of BSA-loaded double-walled glucose-poly(lactideco- glycolide) microspheres. Archives of Pharmacal Research, 39 (9). pp. 1242-1256. ISSN 0253-6269 E-ISSN 1976-3786

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Abstract

The aim of this study was to prepare a model protein, bovine serum albumin (BSA) loaded double-walled microspheres using a fast degrading glucose core, hydroxyl-terminated poly(lactide-co-glycolide) (Glu- PLGA) and a moderate-degrading carboxyl-terminated PLGA polymers to reduce the initial burst release and to eliminate the lag phase from the release profile of PLGA microspheres. The double-walled microspheres were prepared using a modified water-in-oil-in-oil-in-water (w/o/o/w) method and singlepolymer microspheres were prepared using a conventional water-in-oil-in-water (w/o/w) emulsion solvent evaporation method. The particle size, morphology, encapsulation efficiency, thermal properties, in vitro drug release and structural integrity of BSA were evaluated in this study. Double-walled microspheres prepared with Glu-PLGA and PLGA polymers with a mass ratio of 1:1 were non-porous, smooth-surfaced, and spherical in shape. A significant reduction of initial burst release was achieved for the double-walled microspheres compared to single-polymer microspheres. In addition, microspheres prepared using Glu- PLGA and PLGA polymers in a mass ratio of 1:1 exhibited continuous BSA release after the small initial burst without any lag phase. It can be concluded that the double-walled microspheres made of Glu-PLGA and PLGA polymers in a mass ratio of 1:1 can be a potential delivery system for pharmaceutical proteins.

Item Type:	Article (Journal)
Additional Information:	5269/49239
Uncontrolled Keywords:	Drug delivery Controlled release Encapsulation efficiency Microspheres Poly(lactide-co-glycolide) Therapeutic proteins Int
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry Kulliyyah of Science
Depositing User:	Dr Mohd Mokhlesur Rahman
Date Deposited:	25 Feb 2016 16:58
Last Modified:	16 Oct 2017 14:07
URI:	http://irep.iium.edu.my/id/eprint/49239

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