Ionic liquid-tolerant cellulase from palm kernel cake for efficient lignocellulose hydrolysis

Elgharbawy, Amal Ahmed and Alam, Md. Zahangir and Jamal, Parveen and Moniruzzaman, Md. (2015) Ionic liquid-tolerant cellulase from palm kernel cake for efficient lignocellulose hydrolysis. In: Asian Congress on Biotechnology 2015 (ACB 2015), 15th-19th Nov 2015, Istana Hotel, Kuala Lumpur. (Unpublished)

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Abstract

Palm kernel cake (PKC) was utilized as the main media for cellulase enzyme production using solid-state bioconversion (SSB). The fermentation was carried out for 7 days using Trichoderma reesei with 0.05% Tween-80 and moisture content of 62.0% (w/w). The enzyme was extracted with citrate buffer (pH 4.8) at 150 rpm agitation speed. The enzyme extract was centrifuged and then purified by ultrafiltration membrane. The activity of the cellulase was determined by carboxymethyl cellulose (CMC) assay at 72.45 Unit/ml (603.75 U/ g of dry PKC). The PKC-cellulase (PKC-Cel) was tested in several ionic liquids (ILs) for its compatibility and stability as a combined system and the residual activity was calculated by taking the IL-free enzyme as the control (100%). The ionic liquids were: choline acetate (CoAc), 1-ethyl-3-methylimidazolium acetate (EMIMAc), dimethylimidazolium dimethylphosphate 90.45±0.44% of its activity in the concentration of 10.0, 20.0 and 40.0% of the (IL/citrate buffer) solution, where at 60.0 and 80.0%, 76.66±2.46 activity was retained. At a concentration of 100%, 63.16±0.04 of the initial activity was maintained for a duration of 6 hours. On the other hand, at 100% of EMIMAc, PKC-Cel could only sustain 4.75±0.2315% of the initial activity. In contrast, 95.62±0.68% was maintained in EM- DEP in concentration varied at 10, 20 and 40% of the (IL/buffer) whereas 36.03±0.46% remained in the pure IL. In DM-DMP, PKC-Cel was stable with no loss of its activity at 10.0, 20.0 and 40% for the first 3 hours, retaining only 8.17±0.28% of its activity after 6 hours.

Item Type:	Conference or Workshop Item (Abstract)
Additional Information:	4157/47895
Uncontrolled Keywords:	Solid-state bioconversion; Ionic liquids; Palm kernel cake; Trichoderma reesei; Residual activity; Cellulase
Subjects:	T Technology > TP Chemical technology > TP155 Chemical engineering
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr. Md. Zahangir Alam
Date Deposited:	02 Feb 2016 11:13
Last Modified:	02 Feb 2016 11:13
URI:	http://irep.iium.edu.my/id/eprint/47895

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