Scaling-up recombinant enzyme fermentation

Azmi, Azlin Suhaida and Sulaiman, Sarina and Mohamed Azmin, Nor Fadhillah and Ali, Fathilah (2015) Scaling-up recombinant enzyme fermentation. In: Recombinant Enzymes - From Basic Science to Commercialization. Springer International Publishing, Springer International Publishing Switzerland, pp. 99-113. ISBN 978-3-319-12397-4

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Abstract

Bioreactor or fermenter is the heart of a process. Scaling up a bioreactor and maintaining the same optimized process in a recombinant enzyme production in a higher scale is an engineering challenge. Scale-up is about mixing and mass transfer, a combination of art and science. It is more to methodological approach with some combination of rules of thumb, experience and/or trial and error. This chapter presented several bioreactor or fermenter modes and types before encompasses four different scale-up strategies. The scale-up strategies related to mass transfer involving constants scale-up of; (i)volumetric transfer coefficient (KLa), (ii) power input per liquid volume (P/V), (iii) impeller tip speed (Vi), and (iv) mixing time (t m) are described and discussed.

Item Type:	Book Chapter
Additional Information:	5339/47856
Subjects:	T Technology > TA Engineering (General). Civil engineering (General) > TA164 Bioengineering
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr Fathilah Ali
Date Deposited:	27 Jan 2016 16:04
Last Modified:	19 Jul 2016 21:09
URI:	http://irep.iium.edu.my/id/eprint/47856

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