Preparation, characterization and in vitro release studies of insulin-loaded double-walled poly(lactide-co-glycolide) microspheres

Ansary, Md. Rezaul Haque and Rahman, Md. Mokhlesur and Awang, Mohamed and Katas, Haliza and Ab. Hadi, Hazrina and Abd Almonem, Doolaanea (2016) Preparation, characterization and in vitro release studies of insulin-loaded double-walled poly(lactide-co-glycolide) microspheres. Drug Delivery and Translational Research. pp. 1-11. ISSN 2190-393X E-ISSN 2190-3948

	PDF - Accepted Version Restricted to Repository staff only Download (2MB) | Request a copy
	PDF Restricted to Repository staff only Download (113kB) | Request a copy

Abstract

The purpose of this study was to fabricate insulin-loaded double-walled and singlepolymer poly(lactide-co-glycolide) (PLGA) microspheres using a fast degrading glucose core, hydroxyl-terminated poly(lactide-co-glycolide) (Glu-PLGA) and a moderate degrading carboxyl-terminated PLGA polymers. A modified water-in-oil-in-oilin-water (w/o/o/w) emulsion solvent evaporation technique was employed to prepare double-walled microspheres, whereas single-polymer microspheres were fabricated by a conventional water-in-oil-in-water (w/o/w) emulsion solvent evaporation method. The effect of fabrication techniques and polymer characteristics on microspheres size,morphology, encapsulation efficiency, in vitro release and insulin stability were evaluated. The prepared double-walled microspheres were essentially non-porous,smooth surfaced, and spherical in shape, whereas single-polymer microspheres were highly porous. Double-walled microspheres exhibited a significantly reduced initial burst followed by sustained and almost complete release of insulin compared to singlepolymer microspheres. Initial burst release was further suppressed from double-walled microspheres when the mass ratio of the component polymers was increased. In conclusion, double-walled microspheres made of Glu-PLGA and PLGA can be a potential delivery system of therapeutic insulin.

Item Type:	Article (Journal)
Additional Information:	5269/47774
Uncontrolled Keywords:	Double-walled microspheres Poly(lactide-co-glycolide) Sustained release Burst release Encapsulation efficiency
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr Mohd Mokhlesur Rahman
Date Deposited:	23 Feb 2016 08:49
Last Modified:	20 Mar 2017 12:11
URI:	http://irep.iium.edu.my/id/eprint/47774

Actions (login required)

View Item