Differential osteogenic potential of human adipose-derived stem cells co-cultured with human osteoblasts on polymeric microfiber scaffolds

Rozila, Ismail and Azari, Pedram and Munirah, Sha'ban and Wan Safwani, Wan Kamarul Zaman and Gan, Seng Neon and Nur Azurah, Abdul Ghani and Jahendran, Jeevanan and Pingguan-Murphy, Belinda and Chua, Kien Hui (2015) Differential osteogenic potential of human adipose-derived stem cells co-cultured with human osteoblasts on polymeric microfiber scaffolds. Journal of Biomedical Materials Research Part A, 104 (2). pp. 377-387. ISSN 15493296

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Abstract

The osteogenic potential of human adipose-derived stem cells (HADSCs) co-cultured with human osteoblasts (HOBs) using selected HADSCs/HOBs ratios of 1:1, 2:1, and 1:2, respectively, is evaluated. The HADSCs/HOBs were seeded on electrospun three-dimensional poly[(R)23-hydroxybutyric acid] (PHB) blended with bovine-derived hydroxyapatite (BHA). Monocultures of HADSCs and HOBs were used as control groups. The effects of PHB–BHA scaffold on cell proliferation and cell morphology were assessed by AlamarBlue assay and field emission scanning electron microscopy. Cell differentiation, cell mineralization, and osteogenic related gene expression of co-culture HADSCs/HOBs were examined by alkaline phosphatase (ALP) assay, alizarin Red S assay, and quantitative real time PCR, respectively. The results showed that co-culture of HADSCs/HOBs, 1:1 grown into PHB–BHA promoted better cell adhesion, displayed a significant higher cell proliferation, higher production of ALP, extracellular mineralization and osteogenic-related gene expression of run-related transcription factor, bone sialoprotein, osteopontin, and osteocalcin compared to other coculture groups. This result also suggests that the use of electrospun PHB–BHA in a co-culture HADSCs/HOBs system may serve as promising approach to facilitate osteogenic differentiation activity of HADSCs through direct cell-to-cell contact with HOBs.

Item Type:	Article (Journal)
Additional Information:	6006/47514
Uncontrolled Keywords:	human adipose-derived stem cells;human osteoblasts; polyhydroxybutyrate; hydroxyapatite; osteogenesis; electrospun; co-culture system
Subjects:	R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User:	Munirah Sha'ban
Date Deposited:	06 Jan 2016 21:45
Last Modified:	21 May 2018 12:14
URI:	http://irep.iium.edu.my/id/eprint/47514

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