The study of cell attachment and spreading on polyaniline and gelatin using electric cell-substrate impedance sensing

Ibrahim, Irmanisha and Nordin, Anis Nurashikin and Hashim, Yumi Zuhanis Has-Yun (2014) The study of cell attachment and spreading on polyaniline and gelatin using electric cell-substrate impedance sensing. Journal of Pure and Applied Microbiology, 8 (sp.ed). pp. 827-831. ISSN 0973-7510

	PDF - Published Version Restricted to Registered users only Download (384kB) | Request a copy
Preview	PDF Download (465kB) | Preview

Abstract

Electrical cell-substrate impedance sensing (ECIS) is a valuable tool for real time monitoring of cell behavior such as attachment, mobility, and growth. This study investigates the correlation between cell attachment and impedance when cells are attached to two different substrates, polyaniline and gelatin, respectively. Colon cancer cell lines, HCT-116 were used as model cell line. The impedance measurements were measured every 8 hours for 104 hours at frequency of 40Hz to 10MHz using impedance analyzer. Polyaniline produced a graph that is in agreement with typical growth curve for mammalian cell culture at lag, and early log phase. However, gelatin graph showed a different trend. This may be due to the high conductivity of the polyaniline which gave better cell attachment and spreading for the HCT-116 cells. The efficiency of the biosensor was measured by cytotoxicity test using 2.5 μg/ml 5-FU and the changes on impedance were analyzed. In conclusion, the cell attachment correlates with impedance depending on the substrate used to culture the cells. To this end, ECIS is proven as an alternative tool to measure cell behavior with an added advantage of ability to monitor the progress in real-time and showed a great potential in drug testing application.

Item Type:	Article (Journal)
Additional Information:	3239/47157
Uncontrolled Keywords:	Cell attachment; Drug testing; ECIS; HCT-116; Impedance biosensor
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering Kulliyyah of Engineering > Department of Electrical and Computer Engineering
Depositing User:	DR YUMI ZUHANIS HAS-YUN HASHIM
Date Deposited:	10 Apr 2016 14:49
Last Modified:	09 Jan 2018 16:02
URI:	http://irep.iium.edu.my/id/eprint/47157

Actions (login required)

View Item