The effect of dose on the intensity of dependence in morphine-dependent mice

Jusoh, Ahmad Jamaluddin Al-Qasami and Syd Mohmad Faudzi, Syed Mohd Syahmi and Suhaimi, Maryam Saadah and Hashim, Ridzwan and Ridzwan, Irna Elina (2015) The effect of dose on the intensity of dependence in morphine-dependent mice. In: Health and Pharmaceutical Sciences Undergraduate Research Symposium, 10th October, 2015, Le Quadri Ball Room, KL campus, North Wing, UCSI University, KL, Malaysia. (Unpublished)

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Abstract

Introduction: Animal behaviour research is an important tool to study the addiction properties of addictive substances. The Conditioned Place Preference (CPP)paradigm is among the model used to achieve this purpose. It can be used to investigate a wide range of addiction related behaviour including intoxication and relapse potential. Since it involves a long cycle experiment, it is important to optimise the experimental conditions, but minimising any unnecessity. Therefore, in this study we aim to investigate the effects of different morphine dose (3.75 - 10 mg/kg) towards intensity of morphine dependence in adult Swiss albino mice. Methods: The mice were divided into four group (n=10-12 for each group), where each group received different doses of morphine and saline on alternate day for 6 days during conditioning phase. The intensity of dependence was measured during post-conditioning phase where the preference at the drug-paired compartment was calculated during a 15 minutes observation. Results: The result has shown that three out of four groups significantly developed dependence (P < 0.05). However, the group of mice that received highest morphine dose (10 mg/kg) did not develop dependence. When baseline was compared, it was found that the mice that received the lowest morphine treatment (3.75 mg/kg) shows the highest increment from their baseline (164.7±33.3 seconds), while the group that received 10 mg/kg morphine shows the lowest increment (119.0±31.6 seconds). The trend of increment at the drug-paired compartment shows a declining trend when the dose of morphine is increased. Conclusion: Therefore, it is concluded that the optimum dose of morphine that should be administered to the adult Swiss albino mice is around 3.75 mg/kg, provided that all other experimental conditions and protocols are maintained.

Item Type:	Conference or Workshop Item (Slide Presentation)
Additional Information:	5465/45949
Uncontrolled Keywords:	morphine, conditioned placed reference, dependence, addiction
Subjects:	R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy Kulliyyah of Allied Health Sciences
Depositing User:	Dr Irna Elina Ridzwan
Date Deposited:	18 Dec 2015 08:37
Last Modified:	18 Dec 2015 08:37
URI:	http://irep.iium.edu.my/id/eprint/45949

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