Bioassay guided isolation of an antidermatophytic active constituents from the stembarks of Entada spiralis Ridl (Pengasingan komponen aktif antiderma secara berperingkat dari kulit pokok Entada spiralis Ridl.)

Harun, Aiza and Mat So'ad, Siti Zaiton and Mohd Hassan, Norazian (2015) Bioassay guided isolation of an antidermatophytic active constituents from the stembarks of Entada spiralis Ridl (Pengasingan komponen aktif antiderma secara berperingkat dari kulit pokok Entada spiralis Ridl.). Malaysian Journal of Analytical Sciences (MJAS), 19 (4). pp. 752-759. ISSN 1394-2506

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Abstract

Entada spiralis Ridl. (Leguminoceae) is a liana or woody climber that grows in the wild in Malaysia and is locally known as ‘Beluru’ or ‘Sintok’. The isolation and characterization of the chemical constituent from an active fraction have been carried out since no previous study has determined any active components from the stem bark. Our previous study had revealed methanol extract of E. spiralis stem bark exhibited promising antifungal activity against three dermatophytes strains, namely Trichophyton mentagrophytes ATCC 9533, Trichophyton tonsurans ATCC 28942 and Microsporum gypseum ATCC 24102 that cause skin infection. This study was performed to elucidate the structure of active constituent known as ester saponin from the active fraction of E. spiralis stem bark. The fractions were prepared using fractionation process and repeated antifungal test was conducted to identify the most active fraction. The structure elucidation of this compound was based on spectroscopic data (1H,13C NMR, HMQC, HMBC and DEPT135) and comparison with literature. On the basis of spectroscopic analysis, the compound was identified as 28-α,L-rhamnopyranosyl-18,21,22-trihydroxy-12-en-29-(2-acetylamino-β-D-glucopyranosyl) triterpene ester. The current study provides important baseline information for the use of E.spiralis stem bark for the treatment of skin infection caused by the microorganisms investigated in this study. Entada spiralis Ridl. (Leguminoceae) merupakan sejenis pokok memanjat yang tumbuh meliar di Malaysia. Ia dikenali juga dengan nama ‘Beluru’ atau ‘Sintok’ Pemencilan dan pengenalpastian komponen kimia yang aktif telah dilakukan dalam kajian ini memandangkan tiada kajian terdahulu mengenai pengasingan bahan aktif dari kulit batang pokok E. spiralis. Kajian terdahulu hanya melaporkan ekstrak metanol mampu merencatkan pertumbuhan tiga jenis kulat penyakit kulit iaitu Trichophyton mentagrophytes ATCC 9533, Trichophyton tonsurans ATCC 28942 and Microsporum gypseum ATCC 24102. Oleh itu kajian ini dijalankan untuk menentukan struktur molekul dan penamaan komponen aktif dari kulit pokok terhadap kulat penyakit kulit dengan menggunakan kaedah spektroskopi (1H,13C NMR, HMQC, HMBC dan DEPT135) serta perbandingan dengan kajian terdahulu. Melalui analisis spektroskopi komponen aktif tersebut dikenal pasti sebagai 28-α,L-rhamnopyranosyl-18,21,22-trihydroxy-12-en-29-(2-acetylamino-β-D-glucopyranosyl) ester triterpena. Penemuan ini boleh dijadikan maklumat asas dalam rawatan penyembuhan penyakit kulit.

Item Type:	Article (Journal)
Additional Information:	5652/44353
Uncontrolled Keywords:	antifungal activity, dermatophytes, entada spiralis, leguminoceae, terpenoid, aktivi antikulat, dermatofit, entada spiralis, leguminoceae, terpenoid
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr. Siti Zaiton Mat So'ad
Date Deposited:	20 Aug 2015 09:26
Last Modified:	15 Nov 2017 16:48
URI:	http://irep.iium.edu.my/id/eprint/44353

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