Cell proliferation assay and sulphated glycosaminoglycan production in poly(lactic-co-glycolic acid)-based scaffolds seeded with bone marrow mesenchymal stem cells for cartilage tissue engineering

Abdul Rahman, Rozlin and A. M., Noradibah and Azeman, Nor Athirah and Mohamad Sukri, Norhamiza and Md Nazir, Norhidayah and Ahmad Radzi, Muhammad Aa'zamuddin and Mohamad, Mohd Yusof and Zulkifly, Ahmad Hafiz and Che Ahmad, Aminudin and Abdul Rahman, Suzanah and Sha'ban, Munirah (2014) Cell proliferation assay and sulphated glycosaminoglycan production in poly(lactic-co-glycolic acid)-based scaffolds seeded with bone marrow mesenchymal stem cells for cartilage tissue engineering. Regenerative Research, 3 (2). pp. 163-164. ISSN 2232-0822 (Online)

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Abstract

Articular cartilage tissue is known to have poor capability of self-repair. Therefore, tissue engineering has significantly emerged as an alternative solution for articular cartilage restoration. Poly(lactic-co-glycolic acid) (PLGA) and fibrin have been widely used as biocompatible scaffolds materials to regenerate tissue. In this study, bone marrow mesenchymal stem cells (BMMSCs) were seeded onto the PLGA with (PLGA/Fibrin) or without fibrin (PLGA only). Macroscopic observation, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and sulphated glycosaminoglycan (sGAG) assays were performed at week 1, 2 and 3 in vitro. Both PLGA/Fibrin and PLGA only groups showed similar hyaline-like appearance with white and smooth glistening surface. The MTT assay showed higher cell proliferation in the PLGA/Fibrin than the PLGA only group. The production of sGAG is higher in the PLGA/Fibrin group than in the PLGA only. This may suggest that PLGA/Fibrin may serve as prospective cell delivery vehicle and essential foundation for in vitro tissue engineered articular cartilage.

Item Type:	Article (Journal)
Additional Information:	6006/41428
Uncontrolled Keywords:	Articular cartilage, Tissue Engineering, PLGA
Subjects:	R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Medicine > Department of Department of Orthopaedics, Traumatology & Rehabilitation
Depositing User:	Munirah Sha'ban
Date Deposited:	12 Feb 2015 09:28
Last Modified:	01 Feb 2021 09:29
URI:	http://irep.iium.edu.my/id/eprint/41428

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