Expression of segment a of infectious bursal disease virus in pichia pastoris

Ghazali, Mohd. Shahrin and Ahmad Raus, Raha and Amin, Noriha M. and Shohaimi, Syamsiah A. and Abu Bakar, Farah D. and Zainuddin, Norafiza and Jimat, Dzun Noraini and Nor Muhammad, Nor Azlin Alia (2014) Expression of segment a of infectious bursal disease virus in pichia pastoris. Journal of Pure and Applied Microbiology, 8 spl. pp. 875-879. ISSN 0973-7510

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Abstract

Recombinant plasmid containing segment A open reading frame 2 (ORF2) gene of infectious bursal disease virus (IBDV) of a very virulent subtype from local outbreak (strain 3529/92) was constructed. The gene encoding the IBDV structural polyprotein (N-VP2-VP3-VP4-C) was inserted into an expression vector, pPICZ prior to its transformation into Pichia pastoris by electroporation. After the induction of P. pastoris transformant with 0.5% methanol, the production of IBDV polyprotein was observed using Western blot. In P. pastoris, co- or post-translational processing of the large polyprotein occurred, generating a stable C-terminal product (VP3) of correct size, but without any detectable N-terminal product (VP2). The failure to observe the VP2 protein in Western blot analysis was probably due to the conformational epitope problem.

Item Type:	Article (Journal)
Additional Information:	3911/39365
Uncontrolled Keywords:	Segment A, IBDV Expression, Pichia pastoris, Vaccine.
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr Raha Ahmad Raus
Date Deposited:	01 Dec 2014 20:28
Last Modified:	21 Jun 2024 09:44
URI:	http://irep.iium.edu.my/id/eprint/39365

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