Microbial transformation of Clarias gariepinus oil by psuedomonas aeruginosa

Omar, Muhammad Nor and Shaban, Norhayati and Bakar, Latifah Munirah and Zuberdi, Ahmad Muzammil (2014) Microbial transformation of Clarias gariepinus oil by psuedomonas aeruginosa. Oriental Journal of Chemistry, 30 (3). pp. 1147-1151. ISSN 0970 - 020X (P), 2231-5039 (O)

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Abstract

Biotransformation of fatty acid from Malaysian catfish, Clarias gariepinus oil was carried out using Pseudomonas aeruginosa. The lipid from freeze-dried catfish flesh was extracted using a modified Folch method with chloroform-methanol mixture as an extracting solvent. The crude lipid substrate was added to P. aeruginosa culture and incubated for 4 days. After conversion, the products were analyzed by using GC-MS instrument. The result showed that 7,10-dihydroxy-8(E)-octadecenoic acid (DHOD) were abundantly found in the product. The hydroxyl derivative increased while fatty acid contents decreased after biotransformation process. It can be concluded that the bacterial cells had transformed the fatty acids to yield hydroxyl metabolite which can be utilized as starting materials for the bioconversion to pharmaceutical materials.

Item Type:	Article (Journal)
Additional Information:	5626/38627
Uncontrolled Keywords:	Biotransformation, Clarias gariepinus oil, Pseudomonas aeruginosa.
Subjects:	Q Science > QR Microbiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Prof Dr Muhammad Nor Omar
Date Deposited:	08 Oct 2014 09:24
Last Modified:	21 Sep 2017 20:17
URI:	http://irep.iium.edu.my/id/eprint/38627

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