Virgin olive oil phenolics extract inhibit invasion of HT115 human colon cancer cells in vitro and in vivo

Hashim, Yumi Zuhanis Has-Yun and Worthington, Jenny and Allsopp, Philip and Ternan, Nigel G. and Brown, Emma M. and McCann, Mark J. and Rowland, Ian R. and Esposto, Sonia and Servili, Maurizio and Gill, Chris I. R. (2014) Virgin olive oil phenolics extract inhibit invasion of HT115 human colon cancer cells in vitro and in vivo. Food and Function, 5 (7). pp. 1513-1519. ISSN 2042-6496

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Abstract

The decreased cancer risk associated with consumption of olive oilmay be due to the presence of phenolics which can modulate pathways including apoptosis and invasion that are relevant to carcinogenesis. We have previously shown that a virgin olive oil phenolics extract (OVP) inhibited invasion of HT115 colon cancer cells in vitro. In the current study we assessed the in vitro effects of OVP (25 mg mL-1) on HT115 cell migration, spreading and integrin expression. Furthermore, the anti-metastatic activity of OVP – at a dose equivalent to 25 mg per kg per day for 2, 8 or 10 weeks – was assessed in a Severe Combined ImmunoDeficiency (SCID) Balb-c mouse model. After 24 h OVP did not inhibit cell migration but significantly reduced cell spreading on fibronectin (65% of control; p < 0.05) and expression of a range of a and b integrins was modulated. In vivo, OVP by gavage significantly (p < 0.05) decreased not only tumour volume but also the number of metastases in SCID Balb-c mice. Collectively, the data suggest that – possibly through modulation of integrin expression – OVP decreases invasion in vitro and also inhibits metastasis in vivo.

Item Type:	Article (Journal)
Additional Information:	3774/38022
Uncontrolled Keywords:	Virgin olive oil phenolics, inhibit invasion, HT115 human colon cancer cells, vitro, vivo
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	DR YUMI ZUHANIS HAS-YUN HASHIM
Date Deposited:	03 Sep 2014 16:08
Last Modified:	19 Sep 2017 10:52
URI:	http://irep.iium.edu.my/id/eprint/38022

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