HPLC separation and isolation of asiaticoside from Centella asiatica and its biotransformation by A. niger

Alfarra, Helmi Yousif and Omar, Muhammad Nor (2014) HPLC separation and isolation of asiaticoside from Centella asiatica and its biotransformation by A. niger. International Journal of Pharma Medicine and Biological Sciences, 3 (3). pp. 1-8. ISSN 2278-5221

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Abstract

The study was carried out to develop a rapid and accurate HPLC method to isolate the major bioactive phytochemical asiaticoside in Centella asiatica and to transform this metabolite using microorganism. Asiaticoside was separated using an optimized HPLC method using Phenomenex, Luna, 5 μm (C18) (150 mm x 4.6 mm) eluted with Water PH3: Acetonitrile (50:50 V/V). The reported method is easy, fast and accurate, and produced highly pure, resolved isolate with high concentration. Asiaticoside was biologically transformed by Aspergillus niger, and the prescreening results on TLC and 1D and 2D IR showed that the transformed produced has at least two byproducts of the ancestor compound. In addition to that the preliminary screeing showed that the transformed product showed very high cytotoxicity agains HaCat Cells in comprison to the original compound.

Item Type:	Article (Journal)
Additional Information:	5626/37448
Uncontrolled Keywords:	Centella asiatica, Asiaticoside, Biotransformation, Biocatalysis, HPLC, Apergillus niger, 2D IR, HaCat Cells, Cytotoxicity
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Prof Dr Muhammad Nor Omar
Date Deposited:	25 Jul 2014 09:03
Last Modified:	19 Jun 2018 15:34
URI:	http://irep.iium.edu.my/id/eprint/37448

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• HPLC separation and isolation of asiaticoside from Centella asiatica and its biotransformation by A. niger. (deposited 25 Jul 2014 09:03) [Currently Displayed]

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