GC-MS based metabolic profiling of ginger (Z. officinale Rosc.) plantlets

Shapiei, Shazatul Azida and Jameel, Mahdi Harith and Budi Muljono, Julia Retno Andayani (2012) GC-MS based metabolic profiling of ginger (Z. officinale Rosc.) plantlets. In: 4th International Conference on National Products for Health and Beauty (NAPRO 4), 28-30 Nov. 2012, Chiang Mai, Thailand.

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Abstract

Introduction and Objective: Ginger (Zingiber officinale Rosc.) is a valuable plant because of wide application in culinary and medicinal uses. Major pungent constituents of ginger rhizomes namely gingerols and their derivatives such as shogaols and paradols are believed to be responsible for most of their pharmacological activities. The proportion of these compounds might vary considerably due to either environmental or genetic factors. In the present study, we intended to do profiling ginger root metabolites of tissue culture-derived plantlets originated from Bukit Tinggi and Sabah using gas chromatography-mass spectrometry. Method: Sample preparation and MSTFA derivatization of chloroform fraction of lyophilized ginger root were carried out according to Shepherd's method (5) with a slight modification. The GC-MS data were obtained on a Agilent gas chromatograph (6890 N) with a non polar column HP-5MS 5% phenyl methyl siloxane (30 m x 0.25 mm i.d.), film thickness 0.25 µm and interfaced with Mass Detector (MSD). The GC oven and transfer line temperature, 250C; carrier gas helium, (1.2 ml/min), ionization energy, 70 eV. Oven temperature programmed started at 50C for 5 min, to 250 C at 10 C/min till the end of analysis. The peak identity was checked using the Wiley7n.1 spectra data based library as well as NIST Web book. Results: Sequiterpenes hydrocarbon such as -zingiberene, ar-curcumene, -farnacene and -sesquiphellandrene were obtained in the gingerol root plantlets. Te same chemicals are reported to be the most abundance essential oils of gingr rhizomes. In addition a considerable amount of paradola gingerol related compound was also found in the ginger root together with cis-6-shogaol. On the contrary, major constituents of fresh rhizome, gingerol was only found in a trace amount presence in both ginger root plantlets. A similar GC-MS profile of terpenoids and gingerol-relative compounds were detected using a non polar capillary column HP-5MS following a MSTFA derivatization of the analytes. Conclusion: Ginger root plantlets contain a sesquiterpenes -zingiberene and paradol (shogaol related compound) as their major constituents. Apparently, the same artificial growth conditions such as media composition, light intensity and growth temperature applied on giger have resulted in a small vaiability of the ginger metabolites.

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	4790/37372 (ISBN: 9789746727457)
Uncontrolled Keywords:	Gas chromatography-Mass Spectrometry, Ginger (Zingiber officinale Rosc.), Metabolic Profiling, Plant Tissue Culture.
Subjects:	Q Science > Q Science (General) Q Science > QD Chemistry T Technology > T Technology (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr Retno Andayani Budi Muljono
Date Deposited:	08 Aug 2014 14:52
Last Modified:	08 Aug 2014 14:52
URI:	http://irep.iium.edu.my/id/eprint/37372

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