Proteomic analysis and pharmacological activities of the venom of the Moroccan cobra Naja haje legionis

Malih, Ibitissam and Ahmad Rusmili, Muhamad Rusdi and Ting, Yee Tee and Saile, Rachid and Ghalim, Noreddine and Othman, Iekhsan (2012) Proteomic analysis and pharmacological activities of the venom of the Moroccan cobra Naja haje legionis. Toxicon, 60 (2). p. 211. ISSN 0041-0101

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Abstract

Background: In Morocco, envenomation by snake bites poses a serious problem to public health. The cobra Naja haje legionis is endemic of the country and one of the most dangerous species known. In this work, we report the proteomic and pharmacological characterizations of biologically active proteins from the venom of this species. Methods: The various proteins in the crude venom were fractionated and analyzed by a combination of chromatographic separations and proteomic analysis techniques such as gel filtration, RP-HPLC, 1D electrophoresis, in-gel digestion, tandem mass spectrometry and protein database search. The pharmacological properties of Naja haje legionis venom were assessed using in vitro preparations using rodents and chicks. Results: Our venomic strategy allowed the identification of 64 proteins and peptides from known database which can classified into 17 families according to their biological activities. We were able to identify cobra venom factor, L-aminoacid oxidases, acetylcholinesterase, metalloproteinase, disintegrin, cysteine-rich secretory proteins, nerve growth factor, phospholipases A2, vespryns, kunitz-type inhibitor, short neurotoxins, long neurotoxins, weak neurotoxins, neurotoxin like proteins, muscarinic toxins, cytotoxins and cardiotoxins. Pharmacological tests showed that Naja haje legionis venom contained neurotoxic activities inducing irreversible blockage of neuromuscular transmission in both rodent and chick nerve-muscle preparations. This venom also exhibit myotoxic and cardiotoxic activities. Conclusions: These results showed the potency and the complexity of the various proteins present in the venom of Naja haje legionis,which present a very similar pattern to other cobra venoms. The contribution of the different components in venom toxicity deserves further investigations.

Item Type:	Article (Journal)
Additional Information:	5591/37205
Uncontrolled Keywords:	Naja haje legionis, characterization, neurotoxins, proteomics, pharmacology
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Basic Medical Sciences
Depositing User:	Dr Muhamad Rusdi Ahmad Rusmili
Date Deposited:	09 Jul 2014 13:07
Last Modified:	09 Jul 2014 13:07
URI:	http://irep.iium.edu.my/id/eprint/37205

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