Isolation and pharmacological characterization of a short neurotoxin, α-elapitoxin-Bf1b (α-EPTX-Bf1b) from Malaysian Bungarus fasciatus

Ahmad Rusmili, Muhamad Rusdi and Mustafa, Mohd Rais and Othman, Iekhsan and Hodgson, Wayne C. (2013) Isolation and pharmacological characterization of a short neurotoxin, α-elapitoxin-Bf1b (α-EPTX-Bf1b) from Malaysian Bungarus fasciatus. In: XI Congress of the Pan American Section of the International Society on Toxinology and the XII Congress of the Brazilian Society of Toxinology., 8-13 November 2013, Guaruja, Sao Paolo, Brazil. (Unpublished)

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Abstract

Introduction: In Malaysia and other countries in South East Asia, kraits (genus Bungarus) are considered to be a medically important species. The Golden banded krait (Bungarus fasciatus) is one species found in Malaysia. Like many other krait species, Bungarus fasciatus venom has previously been reported to contain a number of postsynaptic neurotoxins that cause blockade of neuronal transmission involving the nicotinic acetylcholine receptor. However, not many of these toxins have been isolated and fully characterized. Here, we would describe the isolation and pharmacological characterization of a novel short neurotoxin that we named α- elapidtoxin-Bf1b. Methods and Results: The toxin was purified by a combination of gel filtration, cation-exchange and reverse-phase chromatography. Based on mass spectrometry and sequencing data, the toxin showed high homology with another toxin, Neurotoxin 3FTx-RI that has previously only been detected in the Bungarus fasciatus venom gland transcriptome. The toxin has an approximate molecular mass of 6.9 kDa and caused concentration-dependent and time-dependent neuromuscular blockade when tested in the indirectly-stimulated chick biventer cervicis muscle preparation. The toxin is also partially reversible after continuous washing. Monovalent Bungarus fasciatus and neurotoxic polyvalent antivenoms were found to be ineffective in reversing the neurotoxicity effect in indirectly-stimulated chick biventer cervicis preparation. Conclusion: We have isolated a novel short neurotoxin from Malaysian Bungarus fasciatus. This toxin is perhaps the first short neurotoxin from Bungarus fasciatus that has been reported to be resistant to both monovalent Bungarus fasciatus and neurotoxic polyvalent antivenoms.

Item Type:	Conference or Workshop Item (Full Paper)
Additional Information:	5591/37204
Uncontrolled Keywords:	neurotoxin
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Basic Medical Sciences
Depositing User:	Dr Muhamad Rusdi Ahmad Rusmili
Date Deposited:	09 Jul 2014 12:43
Last Modified:	09 Jul 2014 12:44
URI:	http://irep.iium.edu.my/id/eprint/37204

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