Human cord blood leukocyte metallothionein-2A MRNA expression is associated with primiparaous and multiparous childbirth

Rahman, Mohammad Tariqur and Youssof, Ayman Lee and Abu Kassim , Noor Lide and Rashid, Siti Aishah and De Ley, Marc (2013) Human cord blood leukocyte metallothionein-2A MRNA expression is associated with primiparaous and multiparous childbirth. In: 38th Annual Conference of the Malaysian Society for Biochemistry and Molecular Biology, 28-29 August 2013, Putrajaya Marriott Hotel and Spa, Putrajaya, Malaysia.

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Abstract

Metallothionein (MT), a metal and stress-induced protein, provides transport and storage of metal ions. The current study analyzed the influence of human cord blood leukocyte [Zn] and gravida on leukocyte MT-2A mRNA expression. Samples (n= 47) were collected at the labor rooms of a Government hospital in Kuantan, Malaysia. [Zn] in plasma, erythrocytes, and leukocytes were measured by AAS (λ=213. 9 nm). MT-2A isoform specific mRNA was quantified by real-time PCR using total leukocyte RNA. Quantification of MT-2A gene transcription was normalized to the housekeeping gene, β-actin. Significant correlations were found between plasma [Zn] and erythrocyte [Zn] (r = .304; p< .05) and between leukocyte [Zn] and plasma [Zn] (r = .295; p< .05). Leukocyte [Zn] exhibited a significant correlation with MT-2A mRNA level (r = .765; p< .001). Higher levels of MT-2A mRNA was found in primiparous mothers (n= 16; M= 1.108, SD= 1.273) compared to multiparous mothers (n= 30; M= 0.581, SD= 0.711). Path analysis showed that leukocyte [Zn] has a greater influence (regression weight .84; p= .000) on MT-2A mRNA expression compared to gravida (regression weight -.18; p= .021). Finally it is concluded that, in addition to circulatory Zn, gravida contributes to the MT-2A mRNA expression. 1, NOOR LIDE 2, 3, MARC DE LEY4, MOHAMMAD TARIQUR 1

Item Type:	Conference or Workshop Item (Full Paper)
Additional Information:	5128/35414
Subjects:	Q Science > QH Natural history > QH301 Biology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Dr Mohammad Tariqur Rahman
Date Deposited:	07 Feb 2014 09:17
Last Modified:	06 Mar 2014 11:25
URI:	http://irep.iium.edu.my/id/eprint/35414

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