Hypoxic culture conditions as a solution for mesenchymal stem cell based regenerative therapy

Haque, Nazmul and Rahman, Mohammad Tariqur and Abu Kasim, Noor Hayaty and Alabsi, Aied Mohammed (2013) Hypoxic culture conditions as a solution for mesenchymal stem cell based regenerative therapy. The Scientific World Journal , 2013 (632972). pp. 1-12. ISSN 1537-744X

PDF - Published Version Restricted to Registered users only Download (1MB) | Request a copy

Abstract

Cell-based regenerative therapies, based on in vitro propagation of stem cells, offer tremendous hope to many individuals suffering from degenerative diseases that were previously deemed untreatable. Due to the self-renewal capacity, multilineage potential, and immunosuppressive property, mesenchymal stem cells (MSCs) are considered as an attractive source of stem cells for regenerative therapies. However, poor growth kinetics, early senescence, and genetic instability during in vitro expansion and poor engraftment after transplantation are considered to be among the major disadvantages of MSC-based regenerative therapies. A number of complex inter- and intracellular interactive signaling systems control growth, multiplication, and differentiation of MSCs in their niche. Common laboratory conditions for stem cell culture involve ambient O2 concentration (20%) in contrast to their niche where they usually reside in 2–9% O2. Notably, O2 plays an important role in maintaining stem cell fate in terms of proliferation and differentiation, by regulating hypoxia-inducible factor-1 (HIF-1) mediated expression of different genes. This paper aims to describe and compare the role of normoxia (20% O2) and hypoxia (2–9% O2) on the biology of MSCs. Finally it is concluded that a hypoxic environment can greatly improve growth kinetics, genetic stability, and expression of chemokine receptors during in vitro expansion and eventually can increase efficiency of MSC-based regenerative therapies. ,1,2 ,3 ,1,2 and

Item Type:	Article (Journal)
Additional Information:	5128/35324
Uncontrolled Keywords:	HUMAN BONE-MARROW; OSTEOGENIC DIFFERENTIATION POTENTIALS; ACUTE MYOCARDIAL-INFARCTION; TISSUE OXYGEN-TENSION; VERSUS-HOST-DISEASE; STROMAL CELLS; PROGENITOR CELLS; GENE-EXPRESSION; OXIDATIVE STRESS; IN-VIVO
Subjects:	R Medicine > RM Therapeutics. Pharmacology > RM270 Serum therapy. Immunotherapy
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Dr Mohammad Tariqur Rahman
Date Deposited:	05 Feb 2014 11:00
Last Modified:	05 Feb 2014 11:00
URI:	http://irep.iium.edu.my/id/eprint/35324

Actions (login required)

View Item