Antiarthritic effects of water extraction of Centella asiatica L. leaves on adjuvant-induced arthritis in rats

Hashim, Ridzwan and Md Radai, Wan Syahirah and Althunibat, Osama Yousef and Zulkipli, Farah Hanis (2013) Antiarthritic effects of water extraction of Centella asiatica L. leaves on adjuvant-induced arthritis in rats. International Journal of Recent Scientific Research , 4 (11). pp. 1701-1705. ISSN 0976-3031

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Abstract

The antiarthritic effect of water extraction of C. asiatica leaves in the adjuvant-induced arthritis in rats was investigated. Prior to this, the chemical constituents in C. asiatica leaves were determined through phytochemical analysis and were found to contain alkaloids, terpenoid, flavonoid and tannin. Leaves of C. asiatica were subjected to water extraction by using blender, filtrated and then freeze-dried to produce dried powder. C. asiatica extracts (CAE) as well as the positive control, dexamethasone showed significant effects when applied to establish adjuvant arthritis. Foot volume of rats was measured by using water displacement method known as plethysmography. Intraperitoneal (i.p) administration of CAE for doses 30 mg/kg and 300 mg/kg b.w. reduced the arthritic edema in the ipsilateral paw of rats with maximal inhibition 54.19 ± 6.43% and 41.05 ± 3.41%, respectively. CAE (doses 30 and 300 mg/kg i.p) was able to prevent the spread of the edema from the ipsilateral to the contralateral paws, which indicated the systemic inhibition. Similar results were observed using steroidal anti-inflammatory agent, dexamethasone (1 mg/kg b.w. i.p). Thus, the findings suggests that the water extract of leaves C. asiatica exerted an antiarthritic activity.

Item Type:	Article (Journal)
Additional Information:	5465/34515
Uncontrolled Keywords:	The antiarthritic effect of water extraction of C. asiatica leaves in the adjuvant-induced arthritis in rats was investigated. Prior to this, the chemical constituents in C. asiatica leaves were determined through phytochemical analysis and were found to contain alkaloids, terpenoid, flavonoid and tannin. Leaves of C. asiatica were subjected to water extraction by using blender, filtrated and then freeze-dried to produce dried powder. C. asiatica extracts (CAE) as well as the positive control, dexamethasone showed significant effects when applied to establish adjuvant arthritis. Foot volume of rats was measured by using water displacement method known as plethysmography. Intraperitoneal (i.p) administration of CAE for doses 30 mg/kg and 300 mg/kg b.w. reduced the arthritic edema in the ipsilateral paw of rats with maximal inhibition 54.19 ± 6.43% and 41.05 ± 3.41%, respectively. CAE (doses 30 and 300 mg/kg i.p) was able to prevent the spread of the edema from the ipsilateral to the contralateral paws, which indicated the systemic inhibition. Similar results were observed using steroidal anti-inflammatory agent, dexamethasone (1 mg/kg b.w. i.p). Thus, the findings suggests that the water extract of leaves C. asiatica exerted an antiarthritic activity
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences
Depositing User:	prof Dr Ridzwan Hashim
Date Deposited:	20 Jan 2014 12:08
Last Modified:	20 Jan 2014 12:08
URI:	http://irep.iium.edu.my/id/eprint/34515

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