Metabolic changes in different developmental stages of vanilla planifolia pods

Palama, Tony Lionel and Khatib, Alfi and Choi, Young Hae and Payet, Bertrand and Fock, Isabelle and Verpoorte, Robert and Kodja, Hippolyte (2009) Metabolic changes in different developmental stages of vanilla planifolia pods. Journal of Agricultural and Food Chemistry, 57 (17). pp. 7651-7658. ISSN 0021-8561

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Abstract

The metabolomic analysis of developing Vanilla planifolia green pods (between 3 and 8 months after pollination) was carried out by nuclear magnetic resonance (NMR) spectroscopy and multivariate data analysis. Multivariate data analysis of the 1H NMR spectra, such as principal component analysis (PCA) and partial least-squares-discriminant analysis (PLS-DA), showed a trend of separation of those samples based on the metabolites present in the methanol/water (1:1) extract. Older pods had a higher content of glucovanillin, vanillin, p-hydroxybenzaldehyde glucoside, p-hydroxybenzaldehyde, and sucrose, while younger pods had more bis[4-(β-D-glucopyranosyloxy)-benzyl]-2-isopropyltartrate (glucoside A), bis[4-(β-D-glucopyranosyloxy)-benzyl]-2-(2-butyl)tartrate (glucoside B), glucose, malic acid, and homocitric acid. A liquid chromatography-mass spectrometry (LC-MS) analysis targeted at phenolic compound content was also performed on the developing pods and confirmed the NMR results. Ratios of aglycones/glucosides were estimated and thus allowed for detection of more minor metabolites in the green vanilla pods. Quantification of compounds based on both LC-MS and NMR analyses showed that free vanillin can reach 24% of the total vanillin content after 8 months of development in the vanilla green pods.

Item Type:	Article (Journal)
Additional Information:	6915/33507
Uncontrolled Keywords:	Vanilla planifolia; vanillin; pod development; metabolomic analysis; nuclear magnetic resonance spectroscopy
Subjects:	S Agriculture > S Agriculture (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr. Alfi Khatib
Date Deposited:	18 Dec 2013 12:08
Last Modified:	18 Dec 2013 12:44
URI:	http://irep.iium.edu.my/id/eprint/33507

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