Discrimination of three Pegaga (Centella) varieties and determination of growth-lighting effects on metabolites content based on the Chemometry of 1H Nuclear Magnetic Resonance spectroscopy

H., Maulidiani and Khatib, Alfi and Shaari, Khozirah and Abas, Faridah and Shitan, Mahendran and Kneer, Ralf and Neto, Victor and Lajis, Nurdin Hj (2012) Discrimination of three Pegaga (Centella) varieties and determination of growth-lighting effects on metabolites content based on the Chemometry of 1H Nuclear Magnetic Resonance spectroscopy. Journal of Agricultural and Food Chemistry, 60 (1). pp. 410-417. ISSN 0021-8561

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Abstract

The metabolites of three species of Apiaceae, also known as Pegaga, were analyzed utilizing 1H NMR spectroscopy and multivariate data analysis. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) resolved the species, Centella asiatica, Hydrocotyle bonariensis, and Hydrocotyle sibthorpioides, into three clusters. The saponins, asiaticoside and madecassoside, along with chlorogenic acids were the metabolites that contributed most to the separation. Furthermore, the effects of growth-lighting condition to metabolite contents were also investigated. The extracts of C. asiatica grown in full-day light exposure exhibited a stronger radical scavenging activity and contained more triterpenes (asiaticoside and madecassoside), flavonoids, and chlorogenic acids as compared to plants grown in 50% shade. This study established the potential of using a combination of 1HNMRspectroscopy and multivariate data analyses in differentiating three closely related species and the effects of growth lighting, based on their metabolite contents and identification of the markers contributing to their differences.

Item Type:	Article (Journal)
Additional Information:	6915/33503
Uncontrolled Keywords:	Pegaga varieties, growth-lighting effects, metabolites content, chemometry, 1H nuclear magnetic resonance
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr. Alfi Khatib
Date Deposited:	18 Dec 2013 11:20
Last Modified:	18 Dec 2013 11:20
URI:	http://irep.iium.edu.my/id/eprint/33503

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