Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor

Mel, Maizirwan and Arifin, Mohd Azmir and Sohif, Hajar Naemah and Syed Hassan, Sharifah (2010) Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor. Medical Journal of Malaysia, 65. pp. 19-21. ISSN 0300-5283

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Abstract

The purpose of this study was to optimize agitation speed, microcarrier concentration and pO2 levels in bioreactor for culturing DF-1cells in DMEM media supplemented with 7% fetal bovine serum. Statistical analysis has shown that the model derived from the results was proven to be significant and pO2 level turned out as the most significant parameter in the experiment. As predicted by analysis, cells grew up to 1.190 x 106 cells/ml when moderate agitation speed, microcarrier concentration, and pO2 level are applied.

Item Type:	Article (Journal)
Additional Information:	4085/3291
Uncontrolled Keywords:	agitation speed, DF-1, DMEM
Subjects:	Q Science > QR Microbiology > QR355 Virology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr Maizirwan Mel
Date Deposited:	22 Sep 2011 12:15
Last Modified:	22 Sep 2011 12:15
URI:	http://irep.iium.edu.my/id/eprint/3291

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