Evaluation of the toxicity of prorocentrum species by liquid chromatography mass-spectrometry and cell-based assay

Caillaud, A. and Canete, E. and Mallat, E. and Fernandez, M. and Mohammad Noor, Normawaty and Moestrup, Ojvind and Franco, J.M. (2006) Evaluation of the toxicity of prorocentrum species by liquid chromatography mass-spectrometry and cell-based assay. In: 12th International Conference on Harmful Algae, 4-8 September 2006, Copenhagen, Denmark.

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Abstract

Dinoflagellates belonging to the genus Prorocentrum were studied for toxicological and toxin production properties by cell assays and liquid chromatographic methods, using fluorescence and mass-spectrometry detection (LC-FD, LC-MS). Cytotoxicity characterised by IC50 and morphological changes was examined. IC50 ranged from 2.103 cell equivalents mL-1 for P. cf faustiae to 133.103 for P. rhathymum. Morphological changes such as rounding and membrane blebbing were observed in fibroblasts exposed to Prorocentrum belizeanum and all Prorocentrum lima from Malaysia suggesting the presence of okadaic acid (OA). Analytical measurements confirmed the production of OA. However toxicity not correlated with the presence of OA suggests the production of derivates of OA or others toxin.

Item Type:	Conference or Workshop Item (UNSPECIFIED)
Additional Information:	6635/32129
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science
Depositing User:	Dr Normawaty Mohammad Noor
Date Deposited:	27 Sep 2013 15:45
Last Modified:	12 May 2016 08:19
URI:	http://irep.iium.edu.my/id/eprint/32129

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