Simultaneous determination of aflatoxins, ochratoxin a, and zearalenone in cereals using a validated HPLC-FLD method and PHRED derivatization system

Rahmani, Anoshep and Selamat, Jinap and Khatib, Alfi and Chin Ping, Tan (2013) Simultaneous determination of aflatoxins, ochratoxin a, and zearalenone in cereals using a validated HPLC-FLD method and PHRED derivatization system. Journal of Liquid Chromatography & Related Technologies, 36 (5). pp. 600-617. ISSN 1082-6076 print/1520-572X online

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Abstract

The objective of the study was to develop and validate an HPLC-FLD method, after sample preparation and derivatization, for the simultaneous determination of aflatoxins (B1, B2, G1, and G2), ochratoxin A (OTA), and zearalenone (ZEA) in cereals. A solid–liquid extraction approach using 80% methanol followed by a multifunctional immunoaffinity AOZ column clean-up was applied for the extraction of these mycotoxins before HPLC analysis. A photochemical reactor enhance derivatization system (PHRED) was used between the HPLC column and fluorescence detector, in order to enhance the fluorescence intensity of aflatoxin B1 and G1. The best chromatographic separation for the simultaneous determination of the nominated mycotoxins was achieved under optimal HPLC conditions. Recoveries were 77.31–104.1% for cereal samples spiked at 6.5,10, and 100 ng=g for total aflatoxins (AFs), OTA, and ZEA, respectively; whereas, the precisions were 2.45–11.14%. Limits of detection (LOD) for AFs, OTA, and ZEA were 0.004–0.012,0.05, and 0.5 ng=g, respectively; whereas. limits of quantification (LOQ) were 0.015–0.05, 0.2, and 2ng=g, correspondingly.

Item Type:	Article (Journal)
Additional Information:	6915/32047
Uncontrolled Keywords:	aflatoxins, cereal, ochratoxin, RP-HPLC, simultaneous determination, zearalenone
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr. Alfi Khatib
Date Deposited:	30 Sep 2013 10:53
Last Modified:	30 Sep 2013 10:53
URI:	http://irep.iium.edu.my/id/eprint/32047

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