LC-MS-MS development and validation for simultaneous quantitation of metformin, glimepiride and pioglitazone in human plasma and its application to a bioequivalence study

Sengupta, Pinaki and Bhaumik, Uttam and Ghosh, Animesh and Sarkar, Amlan Kanti and Chatterjee, Bappaditya and Bose, Anirbandeep and Pal, Tapan Kumar (2009) LC-MS-MS development and validation for simultaneous quantitation of metformin, glimepiride and pioglitazone in human plasma and its application to a bioequivalence study. Chromatographia, 69 (11/12). pp. 1243-1250. ISSN 1612-1112 (O), 0009-5893 (P)

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Abstract

A simple, precise and reproducible liquid chromatography–tandem mass spectrometry method has been developed and validated according to the Food and Drug Administration guidelines for the simultaneous quantitation of antidiabetic drugs metformin, glimiperide and pioglitazone in human plasma using glipizide as an internal standard. Quantitation was performed on a triple quadrupole mass spectrometer employing electrospray ionization technique, operating in multiple reaction monitoring and positive ion mode. Inter-batch and intra-batch coefficient of variation across four validation runs for the quality control samples was less than 7%. The accuracy determined at quality control levels was within 92.81–105.13%. The method was applied to a bioequivalence study.

Item Type:	Article (Journal)
Additional Information:	6923/31908
Uncontrolled Keywords:	Column liquid chromatography Tandem mass spectrometry Pharmacokinetic application Metformin, glimepiride and pioglitazone
Subjects:	R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User:	Dr Bappaditya Chatterjee
Date Deposited:	20 Sep 2013 11:08
Last Modified:	20 Sep 2013 11:10
URI:	http://irep.iium.edu.my/id/eprint/31908

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