Molten Globule-Triggered Inactivation of a thermostable and solvent stable lipase in hydrophilic Solvents

Tengku Abdul Hamid, Tengku Haziyamin and Raja Abd. Rahman, Raja Noor Zaliha and Salleh, Abu Bakar and Basri, Mahiran (2010) Molten Globule-Triggered Inactivation of a thermostable and solvent stable lipase in hydrophilic Solvents. The Protein Journal, 29 (4). pp. 290-297. ISSN 1572-3887

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Abstract

The use of lipase in hydrophilic solvent is usually hampered by inactivation. The solvent stability of a recombinant solvent stable lipase isolated from thermostable Bacillus sp. strain 42 (Lip 42), in DMSO and methanol were studied at different solvent-water compositions. The nzymatic activities were retained in up to45% v/v solvent compositions. The near-UV CD spectra indicated that ertiary structures were perturbed at 60% v/v and above. Far-UV CD in methanol indicated the secondary structure in Lip 42 was retained throughout all solvent compositions. Fluorescence studies indicated formations of molten globules in solvent compositions of 60% v/v and above. The enzyme was able to retain its secondary structures in the presence of methanol; however, there was a general reduction in b-sheet and an increase in a-helix contents. The H-bonding arrangements triggered in methanol and DMSO, respectively, caused different forms of tertiary structure perturbations on Lip 42, despite both showing partial denaturation with molten globule formations.

Item Type:	Article (Journal)
Additional Information:	4261/301
Uncontrolled Keywords:	molten globule, solven stable lipase, hydrophilic solvent, overexpression, thermostable lipase
Subjects:	Q Science > Q Science (General) Q Science > QR Microbiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science
Depositing User:	Dr Tengku Haziyamin Tengku Abd Hamid
Date Deposited:	31 May 2011 11:25
Last Modified:	19 Dec 2011 16:12
URI:	http://irep.iium.edu.my/id/eprint/301

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