Cultivable marine bacterial isolates from a sponge Hyattella cribriformis

C., Prasanna Kumar and John, Akbar and Khan, Ajmal and Lyla, Nair and Yunus, Kamaruzzaman and Khan Chowdhury, Ahmed Jalal (2013) Cultivable marine bacterial isolates from a sponge Hyattella cribriformis. Journal of Biological Sciences, 13 (1). pp. 26-32. ISSN 17273048 (P), 1812-5719 (O)

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Abstract

Cultivable marine bacteria associated with the sponge Hyattella cribriformis (Hyatt, 1877) was studied through 16S rRNA gene sequencing. These marine bacterial colonies produced on Zobell Marine agar plates were distinguished based on phenotypic characters viz., colony morphology and pigmentations of the individual colonies. The extracted DNA from the individual bacterial isolates was PCR amplified using universal 16S primers and subjected to DNA sequencing. BLAST (Basic Local Alignment Tool) analysis finds that Vibrio diazotrophicus, Bacillus subtilis, B. firmus, Thalassomonas agarivorans, Oleiphilus messinensis, Planococcus maritimus and Brevundimonas vesicularis are the culturable marine bacteria associated with the sponge Hyattella cribriformis. The phylogram constructed clearly delineated the bacterial isolates into its corresponding phylum. The cultivable bacterial density of Hyattella cribriformis was found to be 65x10-5 CFU mL-1. Submitted sequences in NCBI were assigned with accession numbers (FJ834327, FJ834328, FJ834329, FJ845392, FJ845393, FJ845394 and FJ845395). It could be concluded that, application of 16S rRNA sequencing approach would yield novel insights into the diversity of bacteria associated with sponges and hence would help in exploring the commercial importance and their industrial applications.

Item Type:	Article (Journal)
Additional Information:	5410/29804
Uncontrolled Keywords:	marine bacterial phylogeny
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Professor Dr. Kamaruzzaman Yunus
Date Deposited:	07 Jun 2013 10:41
Last Modified:	08 Dec 2017 10:37
URI:	http://irep.iium.edu.my/id/eprint/29804

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