Addition of selenium to carica papaya linn pulp extract enhances dermal wound healing activity

Abdulrazaq, Nafiu Bidemi and Akram, Haris and Bero, Dinie Najwa and Yusoff, Mohammed and Abd Malik, Izzati and Rahman, Mohammad Tariqur (2013) Addition of selenium to carica papaya linn pulp extract enhances dermal wound healing activity. Tropical Journal of Pharmaceutical Research, 12 (1). pp. 77-84. ISSN 1596-9827 (O), 1596-5996 (P)

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Abstract

Purpose: To investigate the combined effect of Carica papaya extract and mineral elements wound healing. Methods: Phosphate-buffered saline (PBS) and water extracts of unripe Carica papaya. papaya were analyzed for mineral element composition (Ca, calcium; Mg, magnesium; Na, sodium; Fe, iron; Mn, manganese; Zn, zinc; Ag, silver; and Se, selenium) using a microwave-assisted digestion procedure. Atomic absorption spectrophotometry was used for the analysis of the elements. Phytochemical analysis was conducted using standard protocols. Mice weighing 25 - 30 g were anesthetized, shaved and inflicted with wound at the dorsal region using a biopsy punch. The mice were treated topically twice daily with or without 5 mg/ml PBS or water extracts containing 100 or 200 μM Zn2+ or 1.0 or 0.5 μg/g Se2+. The standard (positive control) group was treated with solcoseryl and the negative control with deionised H2O. Wound area was monitored with a camera and evaluated by software. Results: PBS extract formulated with Se2+. (0.5 or 1.0 mg/g) took approximately 9 days for complete wound healing while negative control took 14 days and other treatment groups least 10 days. PBS extract contained 9 % protein but no tannin while water extract contained 0.58 % protein and tannins. Calcium (137.98 mg/100 g extract) constituted the most abundant macro-element while Se (32.14 mg/100 g extract) was the most abundant microelement in C. papaya pulp. Conclusion: Addition of selenium to C. papaya extracts augments the wound healing activity of the extracts.

Item Type:	Article (Journal)
Additional Information:	4178/29725
Uncontrolled Keywords:	Dermal wound healing; Papaya; Selenium; Zn
Subjects:	Q Science > QM Human anatomy
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biomedical Science
Depositing User:	Dr Mohammad Tariqur Rahman
Date Deposited:	02 Aug 2013 15:35
Last Modified:	05 Sep 2014 16:33
URI:	http://irep.iium.edu.my/id/eprint/29725

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