Interaction between phosphoprotein and nucleocapsid of newcastle disease virus

Ahmad Raus, Raha and Tan, Wen Siang and Ali, Abdul Manaf and Yusoff, Khatijah (2009) Interaction between phosphoprotein and nucleocapsid of newcastle disease virus. In: Malaysian International Conference on Trends in Bioprocess Engineering., 12 - 13 December 2009, Kuala Lumpur.

PDF (full text) - Published Version Restricted to Repository staff only Download (435kB) | Request a copy

Abstract

The phosphoprotein (P) of paramyxoviruses plays a central role in the viral genome RNA replication and transcription. In genome replication, the P binds to the unassembled nucleocapsid (NP0) and activates it to encapsidate the newly synthesized viral genomic RNA. P also plays a supplemental role in genomic RNA synthesis by binding to the ribonucleoprotein template which composed of genomic RNA in complex with the NP. In this study, the P region of Newcastle disease virus (NDV) that binds to assembled NP (NPNC) was mapped. A total of 18 N- and C-terminally truncated P mutants were synthesized by in vitro translation in rabbit reticulocytes containing [35S]-methionine. These radiolabelled mutants were mixed with the purified full-length NP and determined their interactions in immunoprecipitation assay and protein binding assay. Immunoprecipitation assay showed that the region of P that binds to NPNC is located within the internal region of C-terminal half of P, from amino acids 243 to 279. Protein binding assay also showed a similar interactive domain except that the interactive region is slightly bigger (amino acids 224-279). Nevertheless, both assays showed that the Nterminal half and immediate C-terminal end of P is not involved in the binding of P to NPNC.

Item Type:	Conference or Workshop Item (Full Paper)
Additional Information:	3911/29281
Uncontrolled Keywords:	Phosphoprotein, nucleocapsid, immunoprecipitation, protein binding assay
Subjects:	Q Science > Q Science (General) Q Science > QR Microbiology > QR355 Virology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr Raha Ahmad Raus
Date Deposited:	25 Apr 2013 08:54
Last Modified:	25 Jun 2013 19:17
URI:	http://irep.iium.edu.my/id/eprint/29281

Actions (login required)

View Item