Biotransformation of ω-fatty acid from Clarias gariepinus using Psuedomonas aeruginosa

Omar, Muhammad Nor and Shaban, Norhayati and Bakar, Latifah Munirah and Zuberdi, Ahmad Muzammil (2012) Biotransformation of ω-fatty acid from Clarias gariepinus using Psuedomonas aeruginosa. Innova Ciencia, 4 (6). pp. 82-88. ISSN 1333-114X

PDF - Published Version Restricted to Repository staff only Download (627kB) | Request a copy

Abstract

Study on biotransformation of �-fatty acid from fish oil was carried out. Pseudomonas aeruginosa has been chosen to be the biocatalyst for biotransformation of w fatty acids extracted from Malaysian catfish, Clarias gariepinus. The lipid from freeze-dried catfish flesh was extracted using a modified Folch method and the mixture of chloroform and methanol was used as a solvent. The crude lipid substrate was added to the bacterial culture and incubated for 4 days. After conversion, the products were analyzed by using gas chromatography and mass spectrometer (GC-MS). The analytical result showed that several fatty acids and cholesterol were found in the product. However, the fatty acids and cholesterol contents before and after biotransformation were different. The cholesterol content increased while �-fatty acid contents decreased after biotransformation process. It can be concluded that the bacterial cells had oxidized the �-fatty acids to yield precursors which can be utilized as starting materials for the bioconversion to cholesterol.

Item Type:	Article (Journal)
Additional Information:	5626/24742
Uncontrolled Keywords:	biotransformation, Clarias gariepinus, Pseudomonas aeruginosa w fatty acids, cholestrol
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Prof Dr Muhammad Nor Omar
Date Deposited:	13 Aug 2012 09:04
Last Modified:	13 Aug 2012 09:04
URI:	http://irep.iium.edu.my/id/eprint/24742

Actions (login required)

View Item