Some biological activities of Malaysian leech salive extract

M.Abdualkader, Abdualrahman and Merzouk, Ahmed and Mohammed Ghawi, Abbas and Alaama, Mohammed (2011) Some biological activities of Malaysian leech salive extract. IIUM Engineering Journal, 12 (4). pp. 1-9. ISSN 1511-788X

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Abstract

Leeches were fed on the phagostimulatory solution through parafilm membrane. The satiated leeches were forced to regurgitate the solution by soaking them in an ice-container. The anticoagulant activity was ascertained using thrombin time assay (TT). The result revealed that the saliva concentration which increases TT by 100% (IC100) is 43.205 μg/ml plasma. The antimicrobial activity of the saliva was tested against several bacterial spp. (E. coli, P. aeruginosa, B. cereus, Sal. typhi and S. aureus) and fungi spp. (C.albicans and C.neoformans). It was found that saliva has an inhibition activity against Sal. typhi (minimal inhibitory concentration MIC 78.253 μg/ml), S.aureus (MIC 78.253 μg/ml) and E .coli (MIC 121.256 μg/ml).

Item Type:	Article (Journal)
Additional Information:	5834/16870
Uncontrolled Keywords:	leech; anticoagulant; thrombin time; antibacterial activity
Subjects:	R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Basic Medical Sciences
Depositing User:	Mrs Maznah Zakaria
Date Deposited:	25 Jan 2012 08:45
Last Modified:	25 Jan 2012 18:36
URI:	http://irep.iium.edu.my/id/eprint/16870

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