Bioassay-direct fractionation and contact bioautography technique for antimicrobial activities of marine sponge

Darnis, Deny Susanti and Qaralleh, Haitham and Saad, Shahbudin and Syed Osman Idid, Syed Zahir Idid (2010) Bioassay-direct fractionation and contact bioautography technique for antimicrobial activities of marine sponge. In: IIUM Research, Innovation & Invention Exhibition (IRIIE 2010), 26 - 27 January 2010, Kuala Lumpur. (Unpublished)

PDF (Bioassay-direct fractionation and contact bioautography technique for antimicrobial activities of marine sponge.) - Published Version Restricted to Repository staff only Download (1MB)

Abstract

The sponge (1L82008) possessed the highest antimicrobial activity among four sponges and three corals species investigated. The sponge tissue was extracted with methanol and followed with water and examined against two Gram-positive bacteria (Staphylococcus aureus and Bacillus anthracis), two gramnegative bacteria (Escherichia coli and Pseudomonas aeruginosa) and two fungi strains (Candida albicans and Cryptococcus neoformans) using disc diffusion method. The results showed that methanol extract had stronger antimicrobial activity against B. anthracis (400μg/disc) than water extract. Since the methanol extract was found to be active, it was used for further bioassay-directed fraction in order to isolate the active compounds. The dichloromethane and n-butanol fractions were found to be active at concentration 400μg/disc. Purification of n-butanol fraction was done by using normal phase column chromatography eluted with n-butanol:acetic acid:water (12:3:5). Based on thin layer chromatography (TLC) profiles, the same fractions were collected, concentrated and assayed for antimicrobial activity against B. fnthracis. The results showed that fractions (87-123) and fractions (124-183) were the most active fractions. The TLC of the active fractions were then developed using the same mobile phase and subjected to contact bioautography against B. anthracis, which showed inhibition zone at different retention time, indicating the presence of antimicrobial components.

Item Type:	Conference or Workshop Item (Other)
Additional Information:	5641/16567
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biomedical Science Kulliyyah of Science > Institute of Oceanography and Maritime Studies
Depositing User:	Ms Rosyidah Taju Rahim
Date Deposited:	29 Jan 2012 20:28
Last Modified:	29 Jan 2012 20:28
URI:	http://irep.iium.edu.my/id/eprint/16567

Actions (login required)

View Item