Siva Dharma, Danusha and Nasir, Siti Hajjar and Rostam, Muhamad Ashraf and Mohan, Kumeran and Abu Bakar, Noraini (2026) Salivary RANKL and OPG gene expression quantification during intermaxillary elastic traction in orthodontic patients. MethodsX, 17. pp. 1-9. ISSN 22150161
|
PDF
- Published Version
Download (2MB) | Preview |
|
|
PDF (Scopus)
- Supplemental Material
Download (214kB) | Preview |
|
|
PDF (WOS)
- Supplemental Material
Download (416kB) | Preview |
Abstract
This protocol describes a non-invasive workflow for quantifying salivary RANKL and OPG gene expression in orthodontic patients undergoing intermaxillary elastic traction. Unstimulated whole saliva was collected by passive drooling at three predefined time points: baseline before elastic initiation (T0), 24 h (T1), and 7 days (T2), from 30 female orthodontic patients allocated to Class I fixed appliance-only, Class II elastics, and Class III elastics treatment groups. Salivary pellets obtained by sequential centrifugation and physiological saline washing were subjected to column-based total RNA extraction, spectrophotometric quality control, and first-strand cDNA synthesis. Relative gene expression of RANKL and OPG was quantified by RT-qPCR using β-actin as the internal reference gene. Fold-change expression was calculated relative to individual patient baseline values. Group and temporal comparisons were performed using linear mixed models with Bonferroni-corrected pairwise contrasts. This protocol provides sufficient procedural detail for direct replication in prospective cohort investigations of mechanically induced molecular events in orthodontic treatment.
| Item Type: | Article (Journal) |
|---|---|
| Uncontrolled Keywords: | Orthodontic tooth movement OTM biomarkers RT-qPCR Orthodontic elastic Clinical orthodontics |
| Subjects: | R Medicine > RK Dentistry |
| Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): | Kulliyyah of Allied Health Sciences Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Dentistry > Department of Orthodontics |
| Depositing User: | Dr Siti Hajjar Nasir |
| Date Deposited: | 30 Jun 2026 10:21 |
| Last Modified: | 30 Jun 2026 10:21 |
| Queue Number: | 2026-06-Q3739 |
| URI: | http://irep.iium.edu.my/id/eprint/129440 |
Actions (login required)
 |
View Item |