Nik Razi, Nik Ihtisyam Majdah and Mahat, Naji Arafat and Azman, Aida Rasyidah and Abdul Wahab, Roswanira and Oyewusi, Habeebat Adekilekun and Abdul Hamid, Azzmer Azzar and Nordin, Norita (2026) Rhizomucor mieheiLipase nanoconjugates for visualizing latent fingermarks on wet glass slides: bioinformatics, characterization and laboratory assessment. Malaysian Journal of Fundamental and Applied Sciences, 22 (1). pp. 1-22. ISSN 2289-5981 E-ISSN 2289-599X
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Abstract
Developing latent fingermarks on wet, non-porous substrates presents significant challenges, and conventional Small Particle Reagent (SPR) method often involvestoxic components. Existing nanobio-based reagents for fingermark development exhibit a limited fatty acid spectrum. Consequently, investigating the broader ligand specificity of Rhizomucor mieheilipase (RML) nanoconjugate(nanobio-based reagent1, NBR-1) as a potential fingermark biosensor is pertinent.Molecular docking analysis determined the binding affinities of NBR-1 for decanoic, palmitic, docosanoic, and stearic acids to be -4.9, -5.5, -6.1, and -6.8 kcal/mol, respectively. Molecular dynamics simulations, assessed viaroot mean square deviation (RMSD), root mean square fluctuation (RMSF), radius of gyration (Rg), hydrogen bond count, and Molecular Mechanics Poisson-Boltzmann Surface Area (MM-PBSA) analysis, confirmed stable complex formation in all cases, evidenced by consistent hydrogen bonding (distances: 2.2–3.4 Å). NBR-1 characterization by Attenuated Total Reflectance-Fourier Transform Infrared spectroscopy revealed that RML immobilization on F-MWCNTs (NBR-1) was evidenced by a shift of the amide C=O stretch from 1640 to 1653 cm⁻¹ and by the reduced intensity and broadening of the carboxylate C–O peak at 1256 cm⁻¹, confirming polypeptide chain presence.Field Emission Scanning Electron Microscopy demonstrated the increment in the RML thickness, providing evidence for successful RML molecule attachment to the F-MWCNT surface. Under controlled laboratory conditions, the synthesized NBR-1 reagent successfully developed latent fingermarks (exhibiting low background noise) on glass slides submerged in water for periods of 7 and 14 days. These empirical findings corroborate the initial bioinformatic predictions.Therefore, the results robustly validate NBR-1 as a promising candidate technology for visualizing latent fingermarks, specifically on water-immersed, wet non-porous surfaces, in forensic contexts.
| Item Type: | Article (Journal) |
|---|---|
| Uncontrolled Keywords: | Latent fingermarks, Rhizomucor mieheilipase nanoconjugates, nanobio-based reagent, bioinformatics, forensic science. |
| Subjects: | Q Science > QD Chemistry |
| Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): | Kulliyyah of Science Kulliyyah of Science > Department of Biotechnology |
| Depositing User: | AP. Dr. Azzmer Azzar Abdul Hamid |
| Date Deposited: | 11 Mar 2026 12:10 |
| Last Modified: | 11 Mar 2026 12:10 |
| Queue Number: | 2026-03-Q2495 |
| URI: | http://irep.iium.edu.my/id/eprint/127840 |
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