Saharudin, Shahida and Abdullah, Nor Zamzila and A.Talib, Norlelawati and Abd. Fuaat, Azliana and Ab.Rahman, Jamalludin and Buyong, Zunariah (2026) Ultrastructural alterations of liver sinusoidal endothelial cells following chronic monosodium methylarsonate exposure in rats. In: 10th International Virtual Medical Research Symposium, 3 - 4 February 2026, Virtual.
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Abstract
Introduction: Monosodium methylarsonate (MSMA) is an organic arsenical herbicide still used in several developing regions despite limited toxicity data. While most arsenic research focuses on hepatocyte injury, the effects of MSMA on liver sinusoidal endothelial cells (LSEC), a specialized, fenestrated endothelium increasingly recognised for its involvement in early liver injury and toxicant-induced hepatic pathogenesis remain poorly understood. This study aimed to characterize ultrastructural changes in LSEC following chronic MSMA exposure. Materials and method: Twenty-eight male Sprague Dawley rats were assigned to either control or MSMA-treated groups (63.20 mg/kg/day) for 2 or 6 months (n = 7/group). Livers were perfusion-fixed and examined using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). LSEC fenestrations, sinusoidal morphology, and space of Disse features were evaluated descriptively. Results: SEM of control animals showed preserved hepatocyte plates, intact sinusoids, and abundant LSEC fenestrae arranged in sieve plates. MSMA-exposed rats demonstrated reduced fenestral groups at 2 months and more pronounced defenestration with gap formation at 6 months. TEM findings supported these observations, showing fewer hepatocyte microvilli within the space of Disse, loss of the typical attenuated LSEC cytoplasm, chromatin condensation, and occasional caveolae formation. The space of Disse became indistinct in exposed groups, suggesting impaired hepatocyte–sinusoid exchange. Conclusion: Chronic MSMA exposure induces LSEC defenestration and ultrastructural changes consistent with early capillarization, which may represent initial pathogenic events in MSMA-related liver toxicity. Further quantitative fenestration analyses and molecular studies are warranted to elucidate mechanisms of LSEC injury and explore their potential as therapeutic or preventive targets.
| Item Type: | Proceeding Paper (Other) |
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| Uncontrolled Keywords: | Liver sinusoidal endothelial cells, monosodium methylarsonate, defenestration, ultrastructure, electron microscopy |
| Subjects: | Q Science > QM Human anatomy R Medicine > R Medicine (General) |
| Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): | Kulliyyah of Medicine > Department of Basic Medical Kulliyyah of Medicine Kulliyyah of Medicine > Department of Community Medicine (Effective: 1st January 2011) Kulliyyah of Medicine > Department of Pathology & Lab Medicine |
| Depositing User: | DR SHAHIDA SAHARUDIN |
| Date Deposited: | 06 Mar 2026 09:50 |
| Last Modified: | 06 Mar 2026 09:50 |
| Queue Number: | 2026-03-Q2429 |
| URI: | http://irep.iium.edu.my/id/eprint/127764 |
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