Genome editing for enhanced abiotic stress tolerance in selected cereal (poaceae) crops: current applications, tools, and future perspectives

Tamizi, Amin Asyraf and Zainal Abidin, Rabiatul Adawiah and Md Yusof, Anis Afuza and Mohd Zim, Nurul Asyikin and Salleh, Mohd Syahmi and Ahmad Azmi, Nur Sabrina and Zainuddin, Zarina and Harun, Sarahani and Sekeli, Rogayah and Samsulrizal, Nurul Hidayah (2025) Genome editing for enhanced abiotic stress tolerance in selected cereal (poaceae) crops: current applications, tools, and future perspectives. Malaysian Applied Biology, 54 (4). pp. 1-24. ISSN 2462-151X

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Abstract

Recent progress in genome editing (GEd) technology offers an opportunity to accelerate the breeding of improved crops with enhanced resistance and high tolerance to drought and salinity. In this article, we highlight four programmable site-specific nucleases that are considered prominent GEd technologies: meganucleases, zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) nucleases. We then focus on the application of CRISPR/Cas9 system and access the transformation methods that have been used to deliver the system into major cereal crops including rice (Oryza sativa), maize (Zea mays), barley (Hordeum vulgare), sorghum (Sorghum bicolor), and wheat (Triticum aestivum). This review further emphasises the applications of the CRISPR/Cas9 system to impart tolerance to two major abiotic stresses, salinity and drought, in these selected crops. Finally, we summarise bioinformatics tools that are available for cereal genome editing works, including guide RNA (gRNA) design and post-editing analysis tools. This review provides an overview of current progress, identifies research gaps, and offers perspectives for prospective scientists embarking on genome editing in cereals and related crops.

Item Type:	Article (Review)
Uncontrolled Keywords:	Abiotic stress, climate change, crop improvement, omics, plant biotechnology, staple food
Subjects:	S Agriculture > SB Plant culture
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Plant Science Kulliyyah of Science
Depositing User:	Dr Nurul Hidayah Samsulrizal
Date Deposited:	22 Dec 2025 14:39
Last Modified:	22 Dec 2025 14:39
Queue Number:	2025-12-Q1275
URI:	http://irep.iium.edu.my/id/eprint/126384

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