Metallothionein in human thrombocyte precursors, CD61+ megakaryocytes

Rahman, Mohammad Tariqur and De Ley, Marc (2008) Metallothionein in human thrombocyte precursors, CD61+ megakaryocytes. Cell biology and toxicology, 24 (1). pp. 19-25. ISSN 0742-2091 (P), 1573-6822 (O)

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Abstract

The in vitro biosynthesis of metallothionein (MT) was investigated in thrombocyte precursors (megakaryocytes) isolated from human cord blood. Biosynthesis and induction of MT in magnetic cell sorting-separated CD61(+) megakaryocytes was confirmed by immunohistochemical staining using monoclonal mouse anti-MT. The presence of MT was detected both in the nuclear and in the cytoplasmic post-treatment with 100 micromol/L of zinc supplement. Seven isoform-specific mRNAs namely, MT-1A, MT-1B, MT-1E, MT-1G, MT-1H, MT-1X, and MT-2A were detected in the similar area. Using RT-PCR, in vitro upregulation/induction of total MT transcripts was observed in CD61(+) cells at 48 h cell populations left untreated with zinc.

Item Type:	Article (Journal)
Additional Information:	6647/12469
Uncontrolled Keywords:	Cord blood . MACS .MTisogene . Nuclear-MT. Zinc
Subjects:	Q Science > QH Natural history > QH301 Biology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Dr Mohammad Tariqur Rahman
Date Deposited:	29 May 2012 10:50
Last Modified:	07 Dec 2012 07:54
URI:	http://irep.iium.edu.my/id/eprint/12469

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