Optimization of producing whole eyeball sections by routine histology

Abdul Nur, Awatif and Noerdin, Sriviowarti and Yusof, Nurul Asyiqin and Zamli, Zaitunnatakhin (2025) Optimization of producing whole eyeball sections by routine histology. In: 8th International Anatomical Sciences and Cell Biology Conference (IASCBC2025) and the 3rd Malaysian Anatomical Association Conference (MAAC2025), October 2025, Kuala Lumpur, Malaysia.

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Abstract

Introduction: Obtaining high- quality histological sections of the whole eyeball is challenging since the histological process introduces many artefacts, such as retinal detachment, lens cracking, and collapsed eyeball shape. Hence, this study aimed to optimize histological techniques by selecting the best fixative, fixation and lens pre- treatment methods. Methods: Eight mouse eyeballs were divided into six groups and macroscopically assessed. In Phase I, Groups 1–4 underwent fixation with either TB- Fix or 10% neutral buffered formalin (NBF) using immersion or injection techniques. The most effective fixative and fixation methods were then used in Phase II, where Group 5 eyeball was decalcified for 48 hours combined with slurry ice dabbing as the lens pre- treatment, while Group 6 eyeball was immersed in distilled water for the same period, followed by the same lens pre- treatment. The histological qualities of these two groups were then compared to negative controls that underwent only decalcification or no treatment. Results: Results showed that the immersion of TB- Fix, followed by slurry ice lens pre- treatment, significantly enhances the preservation of ocular tissues. Unlike 10% NBF, ethanol and acetic acid in TB- Fix help prevent tissue shrinkage and preserve morphology better than 10% NBF. Additionally, retinal detachment and damage were minimized more effectively with immersion alone rather than combined immersion and intraocular injection techniques. Furthermore, decalcification caused more cracking, whereas dabbing with slurry ice proved superior for lens preservation. Conclusion: Although the present study offers a reliable method, further improvement, especially in lens preservation, is needed to produce high- quality eye histology without any artefacts.

Item Type:	Proceeding Paper (Poster)
Additional Information:	5752/124679
Uncontrolled Keywords:	eye, histology, fixation, lens, retinal detachment
Subjects:	R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Basic Medical Sciences
Depositing User:	Zaitunnatakhin Zamli
Date Deposited:	27 Nov 2025 15:48
Last Modified:	27 Nov 2025 16:17
Queue Number:	2025-11-Q200
URI:	http://irep.iium.edu.my/id/eprint/124679

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