Abu Hanifah, Nabihah and Kuttulebbai Naina Mohamed Salam, Sirajudeen and Abdul Rahim, Roslina and Ahmad Affandi, Khairunisa and Buyong, Zunariah and Abdullah, Nor Zamzila and Ahmad, Hajar Fauzan (2025) Effect of Tualang honey on the regulation of liver lipid metabolizing enzymes gene expression in a 12% high cholesterol diet-induced obese rats. Asia-Pacific Journal of Molecular Biology and Biotechnology, 33 (2 Supplementary). p. 29. ISSN 0128-7451 E-ISSN 2672-7277
|
PDF
- Published Version
Download (488kB) | Preview |
Abstract
Abstract Obesity disrupts liver lipid metabolism by altering lipid-metabolizing enzyme expression. Although treatments for obesity exist, their use is often limited by adverse effects, leading to increasing interest in natural products for treating obesity. Thus, this study aims to investigate the effect of Tualang honey (TH) on liver lipid-metabolizing enzyme gene expression in obese rats due to their beneficial biological properties. Thirty male Sprague-Dawley rats were randomly assigned into five groups (n=6 per group). All rats were initially fed their respective diets for 12 weeks, followed by specific treatments for the subsequent 6 weeks. Group 1 was fed a normal diet (ND) without treatment, while Group 2 received a normal diet supplemented with TH at 3.0 g/kg. Group 3 was given a 12% high-cholesterol diet (HCD) alone, whereas Group 4 received a 12% HCD treated with TH (3.0 g/kg). Lastly, Group 5 was provided with a 12% HCD treated with Orlistat (10 mg/kg). At the end, the rats were sacrificed, livers excised for lipid-metabolizing enzymes gene expression study. Gene expression of acetyl-CoA carboxylase (ACC), sterol regulatory element binding protein-1 (SREBP-1), and HMG-CoA reductase was significantly upregulated, while carnitine palmitoyl transferase-1 (CPT-1) was significantly downregulated in Group 3 compared to Group 1 (p<0.01). When compared to Group 3, TH supplementation in Group 4 significantly downregulated ACC (1.7-fold), SREBP-1 (1.3-fold), and HMG-CoA reductase (1.5-fold) while upregulating CPT-1 expression (0.7-fold) (p<0.01). TH may exert its anti-obesity effects by regulating the expression of genes involved in lipid metabolism.
| Item Type: | Article (Abstract) |
|---|---|
| Uncontrolled Keywords: | lipid metabolism, gene expression, obesity, liver, tualang honey |
| Subjects: | R Medicine > R Medicine (General) |
| Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): | Kulliyyah of Medicine Kulliyyah of Medicine > Department of Basic Medical |
| Depositing User: | Prof.Dr. SIRAJUDEEN KUTTULEBBAI NAINAMOHAMED SALAM |
| Date Deposited: | 11 Jul 2025 09:41 |
| Last Modified: | 11 Jul 2025 09:41 |
| URI: | http://irep.iium.edu.my/id/eprint/122008 |
Actions (login required)
 |
View Item |
Download Statistics
Download Statistics