In vitro analysis of collagen-chitosan scaffold loaded with metronidazole nanoparticle for periodontal disease treatment

Mohd Zayi, Nora Azirah and Halim, Lutfi and Harun @ Ismail, Ahmad Fahmi and Mohamad, Mohd Yusof (2025) In vitro analysis of collagen-chitosan scaffold loaded with metronidazole nanoparticle for periodontal disease treatment. SUSTAINABLE & GREEN MATERIALS. E-ISSN 2996-4369

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Abstract

Periodontal diseases pose significant challenges to oral health, often requiring advanced therapeutic interventions involving the utilization of three-dimensional (3D) scaffolds, However, this intervention could not prevent inflammatory reactions. Hence, recent years have seen a growing emphasis on developing new antibacterial scaffolds in dental tissue engineering. This study aimed to address these challenges by evaluating the antibacterial and biocompatibility properties of a novel collagen-chitosan scaffold loaded with metronidazole nanoparticles (CC-MT) for periodontal therapy. The antibacterial activity of CC-MT against Porphyromonas gingivalis and Fusobacterium nucleatum was assessed using the disk diffusion test. Additionally, cytocompatibility was evaluated by examining cell viability and proliferation using Human Gingival Fibroblast cells (HGF-1), and cell attachment was characterized using filed emission scanning electron microscopy. Our findings revealed that CC-MT exhibited antibacterial effects against the pathogens, with CC-MT (30% metronidazole) showing a higher inhibitory effect, with no significant difference compared to the positive control (metronidazole disc) (p < 0.05). In addition, in vitro cell culture studies revealed that the CC-MT (30% metronidazole) has no negative effect on cell morphology, viability, and proliferation and possesses good biocompatibility. Based on these results, CC-MT demonstrated potential as drug carriers and could be promising candidates for the treatment of periodontal disease.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Periodontal disease; scaffold; metronidazole; nanoparticle
Subjects:	R Medicine > R Medicine (General) R Medicine > RM Therapeutics. Pharmacology > RM147 Administration of Drugs and Other Therapeutic Agents
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Physical Rehabilitation Sciences
Depositing User:	Dr Mohd Yusof Mohamad
Date Deposited:	14 May 2025 12:46
Last Modified:	14 May 2025 12:46
URI:	http://irep.iium.edu.my/id/eprint/120990

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