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A single-reactor system for simultaneous pretreatment and fermentation of POME for bioethanol production

Deb, Nibedita and Rahman, Tawfkur and Alam, Md Zahangir and Miah, Md Shohidullah and Kamal, Reashad (2024) A single-reactor system for simultaneous pretreatment and fermentation of POME for bioethanol production. International Journal of Polymer Science, 2024 (1). pp. 1-21. ISSN 1687-9422 E-ISSN 1687-9430

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Abstract

The objective of this study was to develop an efficient method for enhancing the production of bioethanol in a single-reactor system (SRS) by implementing acid–base pretreatment and enzymatic hydrolysis techniques, thereby eliminating differentiation and removal processes. The aim was to establish a process for bioethanol synthesis using hydrolyzed palm oil mill effluent (POME) and a locally sourced cellulase enzyme. The pretreatment and enzymatic hydrolysis methods were successfully optimized, resulting in a maximum yield of reducing sugars of 26.6 g/L (53.14%). To achieve the highest bioethanol yield, fermentation was carried out using both the one-factor-at-a-time (OFAT) and face-centered central composite design (FCCCD) approaches. The OFAT approach was employed to obtain the maximum bioethanol production, which yielded 6.75% v/v of bioethanol from the hydrolyzed POME using the same bioreactor. In the case of the FCCCD process, the optimal conditions led to a bioethanol production of 7.64% v/v during the fermentation stage. Kinetic analyses of the bioethanol produced revealed a specific growth rate (μ) of 0.198 h−1 and a specific product formation rate (qp) of 0.239 h−1 after 3 days of fermentation. These findings highlight a promising strategy for efficient management of POME through the production of biofuels, which could contribute to the economic growth of the country.

Item Type: Article (Journal)
Additional Information: 4157/115430
Uncontrolled Keywords: bioconversion process; bioethanol production; OFAT; POME; reducing sugar; SRS
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering
Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr. Md. Zahangir Alam
Date Deposited: 01 Nov 2024 15:55
Last Modified: 01 Nov 2024 16:04
URI: http://irep.iium.edu.my/id/eprint/115430

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